ZAG Antibody (1D4): sc-13585

ZAG Antibody (1D4) is a mouse monoclonal IgG1 kappa light chain antibody that detects ZAG protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-ZAG antibody (1D4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZAG protein, also known as Zn-α2-glycoprotein, is a soluble, secreted protein predominantly found in serum and other body fluids such as cerebrospinal fluid, blood plasma, urine, and sweat. One of the critical aspects of ZAG is its role in lipid metabolism; ZAG stimulates lipid degradation in adipocytes, which is particularly important in the context of energy homeostasis and metabolic regulation. Notably, ZAG overexpression has been linked to significant fat loss in patients with advanced cancers, highlighting ZAG′s potential as a biomarker for cachexia and other metabolic disorders. Additionally, ZAG exhibits a unique structure that includes a large groove similar to the peptide binding grooves of class I major histocompatibility complex (MHC) proteins, although ZAG does not bind the class I light chain beta-2-microglobulin. This structural similarity suggests ZAG may play a role in immune modulation, further emphasizing ZAG′s importance in both metabolic and immune responses.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ZAG Antibody (1D4) References:

1. Crystal structure of human ZAG, a fat-depleting factor related to MHC molecules.  |  Sánchez, LM., et al. 1999. Science. 283: 1914-9. PMID: 10206894
2. Peptide mapping of proteins in cerebrospinal fluid utilizing a rapid preparative two-dimensional electrophoretic procedure and matrix-assisted laser desorption/ionization mass spectrometry.  |  Davidsson, P. and Nilsson, CL. 1999. Biochim Biophys Acta. 1473: 391-9. PMID: 10594376
3. [Zn-alpha2-glycoprotein in sweat (author's transl)].  |  Jirka, M. and Blanický, P. 1973. Cas Lek Cesk. 112: 1606-8. PMID: 4773177
4. Nephritogenic glycoprotein. IX. Plasma Zn-alpha2-glycoprotein as a second source of nephritogenic glycoprotein in urine.  |  Shibata, S. and Miura, K. 1982. Nephron. 31: 170-6. PMID: 6896906
5. Alternative splicing gives rise to two novel long isoforms of Zn-alpha 2-glycoprotein, a member of the immunoglobulin superfamily.  |  Uría, JA., et al. 1996. Gene. 169: 233-6. PMID: 8647453
6. Biochemical characterization and crystalization of human Zn-alpha2-glycoprotein, a soluble class I major histocompatibility complex homolog.  |  Sánchez, LM., et al. 1997. Proc Natl Acad Sci U S A. 94: 4626-30. PMID: 9114041
7. Renal handling of Zn-alpha2-glycoprotein as compared with that of albumin and the retinol-binding protein.  |  Ekman, R., et al. 1976. J Clin Invest. 57: 945-54. PMID: 985827

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ZAG Antibody (1D4) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-ZAG Antibody (1D4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ZAG Antibody (1D4) Specifications

Alternate Product Name: AZGP1

Product Clone Name: 1D4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: ZAG

Epitope Species: human

Product Reactivity/Detection: ZAG

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for ZAG Antibody (1D4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin