PDH-E1α Antibody (D-6): sc-377092

PDH-E1α Antibody (D-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects PDH-E1 alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PDH-E1α (D-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The pyruvate dehydrogenase (PDH) complex, which includes the PDH-E1α subunit, is crucial for cellular metabolism as PDH-E1α serves as the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. PDH-E1α subunit is a heterotetramer composed of two alpha and two beta subunits, with the E1 active site located within the E1-α subunit, making PDH-E1α essential for the overall function of the PDH complex. PDH-E1α activity is tightly regulated through phosphorylation and dephosphorylation, which is vital for maintaining metabolic homeostasis. PDH-E1α gene is located on chromosome Xp22-q24, and mutations, such as a 20-bp deletion in the last exon, can lead to PDH deficiency, resulting in severe clinical manifestations including seizures, mental retardation, spasticity, and episodes of lactic acidosis associated with cerebellar ataxia.

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PDH-E1α Antibody (D-6) References:

1. A case of PDH-E1 alpha mosaicism in a male patient with severe metabolic lactic acidosis.  |  Seyda, A., et al. 2001. J Inherit Metab Dis. 24: 551-9. PMID: 11757583
2. PDH-E1alpha dephosphorylation and activation in human skeletal muscle during exercise: effect of intralipid infusion.  |  Pilegaard, H., et al. 2006. Diabetes. 55: 3020-7. PMID: 17065338
3. Pyruvate dehydrogenase deficiency due to a 20-bp deletion in exon II of the pyruvate dehydrogenase (PDH) E1 alpha gene.  |  Chun, K., et al. 1991. Am J Hum Genet. 49: 414-20. PMID: 1907799
4. Characterisation of a cDNA for porcine PDH-E1 alpha and comparison with the human cDNA.  |  Sermon, K., et al. 1990. Nucleic Acids Res. 18: 4925. PMID: 2395657
5. The PI3K/Akt Pathway Regulates Oxygen Metabolism via Pyruvate Dehydrogenase (PDH)-E1α Phosphorylation.  |  Cerniglia, GJ., et al. 2015. Mol Cancer Ther. 14: 1928-38. PMID: 25995437
6. MAPK signalling-induced phosphorylation and subcellular translocation of PDHE1α promotes tumour immune evasion.  |  Zhang, Y., et al. 2022. Nat Metab. 4: 374-388. PMID: 35315437
7. PARylated PDHE1α generates acetyl-CoA for local chromatin acetylation and DNA damage repair.  |  Zhang, J., et al. 2023. Nat Struct Mol Biol. 30: 1719-1734. PMID: 37735618
8. Pyruvate dehydrogenase deficiency caused by a 33 base pair duplication in the PDH E1 alpha subunit.  |  Hansen, LL., et al. 1994. Hum Mol Genet. 3: 1021-2. PMID: 7545958
9. Pyruvate dehydrogenase deficiency.  |  Brown, GK., et al. 1994. J Med Genet. 31: 875-9. PMID: 7853374
10. Mutations in the X-linked E1 alpha subunit of pyruvate dehydrogenase leading to deficiency of the pyruvate dehydrogenase complex.  |  Chun, K., et al. 1993. Hum Mol Genet. 2: 449-54. PMID: 8504306
11. Evolution of mammalian X-linked and autosomal Pgk and Pdh E1 alpha subunit genes.  |  Fitzgerald, J., et al. 1996. Mol Biol Evol. 13: 1023-31. PMID: 8752009
12. Association of cerebral dysgenesis and lactic acidemia with X-linked PDH E1 alpha subunit mutations in females.  |  Otero, LJ., et al. 1995. Pediatr Neurol. 13: 327-32. PMID: 8771169

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PDH-E1α Antibody (D-6) has 79 citations in a variety of scientific publications.

Primary antibodies like Anti-PDH-E1α Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PDH-E1α Antibody (D-6) Specifications

Alternate Product Name: PDHA1

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 31-161

Epitope Target: PDH-E1α

Epitope Species: human

Product Reactivity/Detection: PDH-E1α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 79 citations in various scientific publications

Available Conjugates/Forms for PDH-E1α Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin