Jun B Antibody (C-11) recommended for detection of Jun B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Jun B Antibody (C-11): sc-8051

Jun B Antibody (C-11) is rated 4.3 out of 5 by 10.
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Datasheets
    • Jun B Antibody (C-11) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 210-222 near the C-terminus of Jun B of mouse origin
    • recommended for detection of Jun B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-8051 X, 200 µg/0.1 ml)
    • blocking peptide, sc-8051 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of Jun B (C-11): sc-8051.
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Can you recommend a negative control to use with Jun B Antibody (C-11): sc-8051?

Asked by: jenni
We recommend using the blocking peptide sc-8051 P as a negative control. Antibody binding to antigen may be blocked/competed by pre-absorption with the blocking peptide. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 4
Date published: 2017-01-09
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Rated 3 out of 5 by from SDS-PAGE It worked OK, BUT it did not significantly better than previous one
Date published: 2018-03-30
Rated 4 out of 5 by from Worked good I used on Western Blot for NIH3T3 cells with 1:200 dilution,worked good.
Date published: 2017-12-27
Rated 4 out of 5 by from Must use Santa Cruz secondary Ab We used this antibody several years ago. We requested a new sample and tested on HEK-293 cell lysates using a 1:500 dilution. We used Jackson Immuno Research anti-mouse IgG AF-680 secondary and imaged on the Licor Odyssey imager. No signal was detected. We requested a new sample along with the Santa Cruz secondary Ab (AF-680) that is specific for m-IgG Kappa Light Chain. It showed a nice band just under the 37 kD marker. Generally we have found that although whole IgG abs should still recognize these new mouse monoclonals, we get much better signal with the m-IgG Kappa Light Chain secondaries for most sample abs we have tested in both IHC and WB.
Date published: 2017-10-12
Rated 2 out of 5 by from cytoplasm staining Produced immunofluorescence cytoplasm staining in methanol-fixed HK2 cells. why?
Date published: 2017-08-18
Rated 5 out of 5 by from Outstanding product Strong product for multiple applications; best available jun b antibody
Date published: 2017-01-10
Rated 5 out of 5 by from Good Antibody for IHC Versatile antibody showing great staining in human tissue samples as well as strong signal in WB and ELISA assays. Usable in human, mouse, and rat samples for a variety of applications.
Date published: 2016-12-20
Rated 5 out of 5 by from Produced beautiful immunofluorescence nuclear Produced beautiful immunofluorescence nuclear staining in methanol-fixed KNRK cells. -SCBT QC
Date published: 2015-05-01
Rated 5 out of 5 by from Worked great in our blot Worked great in our blot. We tested human
Date published: 2015-04-09
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