Jun B Antibody (C-11) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
specific for an epitope mapping between amino acids 210-222 near the C-terminus of Jun B of mouse origin
recommended for detection of Jun B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM
TransCruz reagent for Gel Supershift and ChIP applications (sc-8051 X, 200 µg/0.1 ml)
blocking peptide, sc-8051 P
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact us to receive a FREE 10 µg sample of Jun B (C-11): sc-8051.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein, along with c-Fos, is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Two additional genes, Jun B and Jun D, have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Can you recommend a negative control to use with Jun B Antibody (C-11): sc-8051?
Asked by: jenni
We recommend using the blocking peptide sc-8051 P as a negative control. Antibody binding to antigen may be blocked/competed by pre-absorption with the blocking peptide. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Rated 3 out of
SDS-PAGEIt worked OK, BUT it did not significantly better than previous one
Date published: 2018-03-30
Rated 4 out of
Worked goodI used on Western Blot for NIH3T3 cells with 1:200 dilution,worked good.
Date published: 2017-12-27
Rated 4 out of
Must use Santa Cruz secondary AbWe used this antibody several years ago. We requested a new sample and tested on HEK-293 cell lysates using a 1:500 dilution. We used Jackson Immuno Research anti-mouse IgG AF-680 secondary and imaged on the Licor Odyssey imager. No signal was detected. We requested a new sample along with the Santa Cruz secondary Ab (AF-680) that is specific for m-IgG Kappa Light Chain. It showed a nice band just under the 37 kD marker. Generally we have found that although whole IgG abs should still recognize these new mouse monoclonals, we get much better signal with the m-IgG Kappa Light Chain secondaries for most sample abs we have tested in both IHC and WB.
Date published: 2017-10-12
Rated 2 out of
cytoplasm stainingProduced immunofluorescence cytoplasm staining in methanol-fixed HK2 cells. why?
Date published: 2017-08-18
Rated 5 out of
Outstanding productStrong product for multiple applications; best available jun b antibody
Date published: 2017-01-10
Rated 5 out of
Good Antibody for IHCVersatile antibody showing great staining in human tissue samples as well as strong signal in WB and ELISA assays. Usable in human, mouse, and rat samples for a variety of applications.
Date published: 2016-12-20
Rated 5 out of
Produced beautiful immunofluorescence nuclearProduced beautiful immunofluorescence nuclear staining in methanol-fixed KNRK cells. -SCBT QC
Date published: 2015-05-01
Rated 5 out of
Worked great in our blotWorked great in our blot. We tested human
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