p-c-Jun Antibody (KM-1) recommended for detection of c-Jun p39 phosphorylated on Serine 63 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and FCM

p-c-Jun Antibody (KM-1): sc-822

p-c-Jun Antibody (KM-1) is rated 4.7 out of 5 by 33.
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Datasheets
    • p-c-Jun Antibody (KM-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • recommended for detection of c-Jun p39 phosphorylated on Serine 63 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and FCM; also reactive with additional species, including and canine, bovine, porcine and avian; non cross-reactive with Jun B or Jun D phosphorylated on the analogous serine residues or with c-Jun non-phosphorylated at Serine 63
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either PerCP, PerCP-Cy5.5 or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-822 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of p-c-Jun (KM-1): sc-822.
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Is there any data to support the fact that this antibody is specific for phosphorylated c-Jun?

Asked by: AbPolly
Thank you for your inquiry. Yes, in Western Blot we see upregulation of signal after the sample is treated with UV radiation, and downregulation of signal following treatment with lambda phosphatase.
Answered by: Technical Service
Date published: 2016-12-22

What is the recommended blocking buffer to use with this antibody in Western Blot?

Asked by: AbPolly
Thank you for your question. When detecting phosphorylated proteins in Western Blot, we recommend using Blotto B as the blocking buffer, sc-2335.
Answered by: Technical Service
Date published: 2016-12-22
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Rated 4 out of 5 by from Great for detection of phosphorylated C-Jun This antibody worked well for the detection of downstream P-C-Jun in the murine macrophage cell line RAW 246. It has minimal background.
Date published: 2018-12-17
Rated 2 out of 5 by from It does not work well We test this ab by 1:500 dilution, and it did not work out well
Date published: 2018-04-03
Rated 4 out of 5 by from good antibody I received this free sample 2 months ago and tested it by Western blot. It shows clean bands in both whole cell lysate and nuclear extract.
Date published: 2018-01-04
Rated 5 out of 5 by from Workable The target band could be detective with this antibody at 1:500 dilution in HepG2.2.15. Great features.
Date published: 2017-10-07
Rated 4 out of 5 by from good antibody You can see target bands with this antibody. The antibody is diluted at 1:2000. The sample is human cell lines treated with a drug.
Date published: 2017-09-30
Rated 4 out of 5 by from Does the job. Robust signal detectable with a 1:500 dilution on western blot.
Date published: 2017-09-13
Rated 5 out of 5 by from p-c-Jun antibody This p-c-Jun antibody worked excellently in our hands.
Date published: 2017-09-13
Rated 5 out of 5 by from good comment Tested in NIH/3T3 cell lysates, diluted in 1:200, the result is very satisfactory.
Date published: 2017-09-05
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