β-Actin (C4): sc-47778. Near-infrared western blot analysis of β-Actin expression in MCF7 (A), NIH/3T3 (B) and KNRK (C) whole cell lysates. Blocked with UltraCruz® Blocking Reagent: sc-516214. Detection reagent used: m-IgGκ BP-CFL 790: sc-516181. Cruz Marker Molecular Weight Standards detected with Cruz Marker MW Tag: sc-516729 and and m-IgGκ BP-CFL 790: sc-516181.
Cruz Marker™ MW Tag: sc-516729
- mouse monoclonal IgG (kappa light chain) provided at 500 µl volume, sufficient for 50 gels
- unconjugated Cruz Marker™ Molecular Weight Standards detection antibody
- recommended for detection of Cruz Marker™ Molecular Weight Standards by chemiluminescent or Near-Infrared WB
- for chemiluminescent visualization, use m-IgGκ BP-HRP (sc-516102)
- for near-infrared visualization, use m-IgGκ BP-CFL 680 (sc-516180) or m-IgGκ BP-CFL 790 (sc-516181)
- this reagent must be used in combination with Cruz Marker™ Molecular Weight Standards (sc-2035), available only from Santa Cruz Biotechnology, Inc.
- The Cruz Marker™ Molecular Weight Standards ladder consists of six bands which appear on the final Western blot image: 132 kDa, 90 kDa, 55 kDa, 43 kDa, 34 kDa, 23 kDa
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Cruz Marker™ MW Tag Antibody (). This work is in progress.
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Cruz Marker™ Molecular Weight Standards use a proprietary technology to allow researchers to visualize the final molecular weight ladder of six bands clearly and consistently on the final Western blot image. Ideal for use in combination with our Prestained Molecular Weight Markers (Broad, High and Low range: sc-2361, sc-2362, sc-2360) for verification and calibration of protein standard migration in SDS PAGE gels and Western blotting membranes.
Cruz Marker™ MW Tag detection antibodies include: Cruz Marker™ MW Tag-Alexa Fluor® 488, Cruz Marker™ MW Tag-Alexa Fluor® 647, Cruz Marker™ MW Tag-Alexa Fluor® 680, Cruz Marker™ MW Tag-Alexa Fluor® 790, Cruz Marker™ MW Tag-HRP and unconjugated Cruz Marker™ MW Tag.
Cruz Marker™ compatible binding proteins include: m-IgGκ BP-HRP (CM) and m-IgGλ BP-HRP (CM).
Cruz Marker™ compatible monoclonal secondary antibodies include: mouse anti-goat IgG-HRP (CM) and mouse anti-rabbit IgG-HRP (CM).
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Cruz Marker™ MW Tag References:
- High-throughput SNP genotyping with the Masscode system. | Kokoris, M., et al. 2000. Mol Diagn. 5: 329-40. PMID: 11172497
- A new approach for fluorescence correlation spectroscopy (FCS) based immunoassays. | Mayboroda, OA., et al. 2004. J Biotechnol. 107: 185-92. PMID: 14711501
- Trilinolein identified as a sex-specific component of tergal glands in alates of Coptotermes formosanus. | Bland, JM., et al. 2004. J Chem Ecol. 30: 835-49. PMID: 15260227
- Serological mapping of the TAG-72 tumor-associated antigen using 19 distinct monoclonal antibodies. | Kuroki, M., et al. 1990. Cancer Res. 50: 4872-9. PMID: 1696162
- Improved non-chromatographic purification of a recombinant protein by cationic elastin-like polypeptides. | Lim, DW., et al. 2007. Biomacromolecules. 8: 1417-24. PMID: 17407348
- Characterization of the shed form of the human tumor-associated glycoprotein (TAG-72) from serous effusions of patients with different types of carcinomas. | Katari, RS., et al. 1990. Cancer Res. 50: 4885-90. PMID: 2379152
- Milk fat triacylglycerols and their relations with milk fatty acid composition, DGAT1 K232A polymorphism, and milk production traits. | Tzompa-Sosa, DA., et al. 2016. J Dairy Sci. 99: 3624-3631. PMID: 26971154
- Effective purification of recombinant peptide ligands for GPCR research. | Ashokan, A. and Aradhyam, GK. 2017. Methods Cell Biol. 142: 111-118. PMID: 28964330
- Seasonal variation in fatty acid and triacylglycerol composition of bovine milk fat. | Pacheco-Pappenheim, S., et al. 2021. J Dairy Sci. 104: 8479-8492. PMID: 34024603
- Composition and interfacial properties play key roles in different lipid digestion between goat and cow milk fat globules in vitro. | Zhao, L., et al. 2022. Food Chem. 374: 131538. PMID: 34839970
- Feeding hydrogenated palm fatty acids and rumen-protected protein to lactating Holstein-Friesian dairy cows modifies milk fat triacylglycerol composition and structure, and solid fat content. | Pacheco-Pappenheim, S., et al. 2022. J Dairy Sci. 105: 2828-2839. PMID: 35181128
- HA-tag CD63 is a novel conditional transgenic approach to track extracellular vesicle interactions with sperm and their transfer at conception. | Morgan, CP., et al. 2023. Sci Rep. 13: 707. PMID: 36639735
- Analysis of a human tumor-associated glycoprotein (TAG-72) identified by monoclonal antibody B72.3. | Johnson, VG., et al. 1986. Cancer Res. 46: 850-7. PMID: 3940648
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cruz Marker™ MW Tag | sc-516729 | 500 µl | $77.00 |