Cruz Marker™ Molecular Weight Standards: sc-2035
- Recommended use is 2-4 µl per lane in SDS-PAGE
- Each vial contains 200 µl volume, sufficient for 50 gels
- The ladder consists of six bands which appear on the final Western blot image: 132 kDa, 90 kDa, 55 kDa, 43 kDa, 34 kDa, 23 kDa
- This reagent must be used in combination with Cruz Marker™ MW Tag detection antibodies available only from Santa Cruz Biotechnology, Inc.
- Cruz Marker™ MW Tag detection antibodies include: Cruz Marker™ MW Tag-Alexa Fluor® 488, Cruz Marker™ MW Tag-Alexa Fluor® 647, Cruz Marker™ MW Tag-Alexa Fluor® 680, Cruz Marker™ MW Tag-Alexa Fluor® 790, Cruz Marker™ MW Tag-HRP and unconjugated Cruz Marker™ MW Tag.
- Cruz Marker™ Molecular Weight Standards appear only on the final Western blot image after incubation with a Cruz Marker™ MW Tag detection antibody. These are not prestained markers.
- Cruz Marker™ Molecular Weight Standards are provided ready to use in SDS-PAGE loading buffer and can be loaded directly into an SDS-PAGE gel. No dilution or reconstitution is required.
- Cruz Markers™ are provided for use as internal molecular weight standards for chemiluminescent and Near-Infrared Western blotting applications and are ideal for approximating the molecular weight of target proteins.
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Cruz Marker™ Molecular Weight Standards use a proprietary technology to allow researchers to visualize the final molecular weight ladder of six bands clearly and consistently on the final Western blot image. Ideal for use in combination with our Prestained Molecular Weight Markers (Broad, High and Low range: sc-2361, sc-2362, sc-2360) for verification and calibration of protein standard migration in SDS PAGE gels and Western blotting membranes.
Cruz Marker™ MW Tag detection antibodies include: Cruz Marker™ MW Tag-Alexa Fluor® 488, Cruz Marker™ MW Tag-Alexa Fluor® 647, Cruz Marker™ MW Tag-Alexa Fluor® 680, Cruz Marker™ MW Tag-Alexa Fluor® 790, Cruz Marker™ MW Tag-HRP and unconjugated Cruz Marker™ MW Tag.
Cruz Marker™ compatible binding proteins include: m-IgGκ BP-HRP (CM) and m-IgGλ BP-HRP (CM).
Cruz Marker™ compatible monoclonal secondary antibodies include: mouse anti-goat IgG-HRP (CM) and mouse anti-rabbit IgG-HRP (CM). Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
Cruz Marker™ Molecular Weight Standards References:
- Measurement of high-molecular-weight hyaluronan in solid tissue using agarose gel electrophoresis. | Armstrong, SE. and Bell, DR. 2002. Anal Biochem. 308: 255-64. PMID: 12419337
- Estimation of molecular weight by polyacrylamide gel electrophoresis using heat stable fluorophors. | Barger, BO., et al. 1976. Anal Biochem. 70: 327-35. PMID: 1267127
- Mesoporous silica chips for selective enrichment and stabilization of low molecular weight proteome. | Bouamrani, A., et al. 2010. Proteomics. 10: 496-505. PMID: 20013801
- Chemical and molecular weight analyses of prosthodontic soft polymers. | Jones, DW., et al. 1991. J Dent Res. 70: 874-9. PMID: 2022770
- Proposal of a laboratory course dedicated to the generation of protein molecular weight standards for sodium dodecyl sulfate-polyacrylamide gel electrophoresis. | Bubis, J. 2021. Biochem Mol Biol Educ. 49: 353-360. PMID: 33301651
- Ceroid lipofuscinosis in sheep. II. The major component of the lipopigment in liver, kidney, pancreas, and brain is low molecular weight protein. | Palmer, DN., et al. 1986. J Biol Chem. 261: 1773-7. PMID: 3944108
- [Studies on the molecular weight standards for size-exclusion chromatography--standardization of a molecular weight standard for water-soluble polymers]. | Yomota, C. and Okada, S. 1997. Kokuritsu Iyakuhin Shokuhin Eisei Kenkyusho Hokoku. 213-5. PMID: 9641850
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cruz Marker™ Molecular Weight Standards | sc-2035 | 200 µl | $115.00 |