CD3 Antibody (PC3/188A): sc-20047

CD3 Antibody (PC3/188A) is a mouse monoclonal IgG1 kappa light chain antibody designed to recognize CD3 in mouse, rat, and human samples, and shows high effectiveness in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and flow cytometry (FCM). CD3, also known as the T cell co-receptor complex or TCR-CD3 complex, functions as an essential component of T cell receptor (TCR) signaling machinery on T lymphocyte surfaces. Structurally, CD3 consists of five invariant polypeptide chains - gamma, epsilon, delta, zeta, and eta - assembling into three distinct dimers: γε, δε, and ζζ or ζη. Post-translational modifications, particularly phosphorylation of immunoreceptor tyrosine-based activation motifs (ITAMs) within gamma, epsilon, delta, and zeta chains, play a crucial role in CD3 functionality. These phosphorylated ITAMs create docking sites for protein kinases like ZAP-70 and Syk, which drive intracellular signaling cascades leading to T cell activation and proliferation. Precise phosphorylation patterns and structural integrity of CD3 enable effective signal transduction, making these modifications key to understanding T cell responses and immune regulation. CD3 monoclonal antibody (PC3/188A) serves as an essential tool for immunological studies, disease modeling, and therapeutic development.

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CD3 Antibody (PC3/188A) References:

1. Structure, assembly and intracellular transport of the T cell receptor for antigen.  |  Exley, M., et al. 1991. Semin Immunol. 3: 283-97. PMID: 1686832
2. Efficiency of T-cell receptor expression in dual-specific T cells is controlled by the intrinsic qualities of the TCR chains within the TCR-CD3 complex.  |  Heemskerk, MH., et al. 2007. Blood. 109: 235-43. PMID: 16968899
3. Structure of the T cell antigen receptor (TCR): two CD3 epsilon subunits in a functional TCR/CD3 complex.  |  de la Hera, A., et al. 1991. J Exp Med. 173: 7-17. PMID: 1824636
4. Signal transduction by the T cell antigen receptor.  |  Weiss, A., et al. 1991. Semin Immunol. 3: 313-24. PMID: 1839225
5. Piecing together the family portrait of TCR-CD3 complexes.  |  Kuhns, MS. and Badgandi, HB. 2012. Immunol Rev. 250: 120-43. PMID: 23046126
6. The Biodistribution of a CD3 and EpCAM Bispecific T-Cell Engager Is Driven by the CD3 Arm.  |  Suurs, FV., et al. 2020. J Nucl Med. 61: 1594-1601. PMID: 32284393
7. Signal transduction. Zapping tandem SH2 domains.  |  Weiss, A. 1995. Nature. 377: 17-8. PMID: 7659151
8. Molecular basis for interaction of the protein tyrosine kinase ZAP-70 with the T-cell receptor.  |  Hatada, MH., et al. 1995. Nature. 377: 32-8. PMID: 7659156
9. Binding of ZAP-70 to phosphorylated T-cell receptor zeta and eta enhances its autophosphorylation and generates specific binding sites for SH2 domain-containing proteins.  |  Neumeister, EN., et al. 1995. Mol Cell Biol. 15: 3171-8. PMID: 7760813
10. Different cytoplasmic structure of the CD3 zeta family dimer modulates the activation signal and function of T cells.  |  Aoe, T., et al. 1994. Int Immunol. 6: 1671-9. PMID: 7865460
11. The role of protein tyrosine kinases and protein tyrosine phosphatases in T cell antigen receptor signal transduction.  |  Chan, AC., et al. 1994. Annu Rev Immunol. 12: 555-92. PMID: 8011291
12. Targeted disruption of the CD3 eta locus causes high lethality in mice: modulation of Oct-1 transcription on the opposite strand.  |  Ohno, H., et al. 1994. EMBO J. 13: 1157-65. PMID: 8131747

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD3 Antibody (PC3/188A) has 154 citations in a variety of scientific publications.

Primary antibodies like Anti-CD3 Antibody (PC3/188A) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD3 Antibody (PC3/188A) Specifications

Alternate Product Name: CD3

Product Clone Name: PC3/188A

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 156-168

Epitope Target: CD313E0GL0RK4

Epitope Species: human

Product Reactivity/Detection: CD3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 154 citations in various scientific publications

Available Conjugates/Forms for CD3 Antibody (PC3/188A): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin