tuberin Antibody (E-9): sc-365103

Tuberin Antibody (E-9) is a mouse monoclonal IgG1 antibody that detects tuberin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Tuberin, a protein consisting of 1784 amino acids, plays a crucial role in the regulation of cell growth and proliferation, making tuberin vital for maintaining cellular homeostasis. Tuberin is primarily localized in membrane fractions, where tuberin interacts with various signaling pathways, including the Ras signaling cascade, to modulate the intrinsic GTPase activity of Rap1A. This interaction is significant because tuberin helps control cellular responses to growth factors and stress, thereby influencing tumorigenesis and other cellular processes. Mutations in the TSC2 gene, which encodes tuberin, are associated with tuberous sclerosis, a genetic disorder characterized by the formation of benign tumors and neurological issues. The TSC2 gene is located on human chromosome 16, and TSC2′s mouse homolog is found on chromosome 17, highlighting the evolutionary conservation of tuberin. Anti-tuberin antibody (E-9) is an essential tool for researchers studying the molecular mechanisms underlying tuberous sclerosis and related pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

tuberin Antibody (E-9) References:

1. Hamartin and tuberin modulate gene transcription via beta-catenin.  |  Jozwiak, J. and Wlodarski, P. 2006. J Neurooncol. 79: 229-34. PMID: 16552619
2. Tuberin activates the proapoptotic molecule BAD.  |  Freilinger, A., et al. 2006. Oncogene. 25: 6467-79. PMID: 16702951
3. Tuberin nuclear localization can be regulated by phosphorylation of its carboxyl terminus.  |  York, B., et al. 2006. Mol Cancer Res. 4: 885-97. PMID: 17114346
4. The tuberous sclerosis gene products hamartin and tuberin are multifunctional proteins with a wide spectrum of interacting partners.  |  Rosner, M., et al. 2008. Mutat Res. 658: 234-46. PMID: 18291711
5. Role of tuberin in neuronal degeneration.  |  Habib, SL., et al. 2008. Neurochem Res. 33: 1113-6. PMID: 18320306
6. Tuberin and mTOR, a key apoptotic pathway in diabetes.  |  Habib, SL. 2011. Cell Cycle. 10: 2237-8. PMID: 21673498
7. Tuberin Regulates Prostaglandin Receptor-Mediated Viability, via Rheb, in mTORC1-Hyperactive Cells.  |  Li, C., et al. 2017. Mol Cancer Res. 15: 1318-1330. PMID: 28710231
8. Nox4 is a Target for Tuberin Deficiency Syndrome.  |  Shi, Q., et al. 2018. Sci Rep. 8: 3781. PMID: 29491408
9. Gene therapy for tuberous sclerosis complex type 2 in a mouse model by delivery of AAV9 encoding a condensed form of tuberin.  |  Cheah, PS., et al. 2021. Sci Adv. 7: PMID: 33523984
10. Beyond Protein Synthesis; The Multifaceted Roles of Tuberin in Cell Cycle Regulation.  |  Fidalgo da Silva, E., et al. 2021. Front Cell Dev Biol. 9: 806521. PMID: 35096832

The preferred p-tuberin antibody is p-tuberin Antibody (325.Ser 1798): sc-293149.