p-ERK 5 Antibody (1.T218/Y220): sc-135760

p-ERK 5 Antibody (1.T218/Y220) is a mouse monoclonal IgG1 kappa light chain antibody that detects dually phosphorylated ERK 5, specifically at Thr 218 and Tyr 220, in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). anti-ERK 5 antibody (1.T218/Y220) is offered in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ERK 5 plays a crucial role in cellular signaling pathways, particularly in response to growth factors and stress signals, and is involved in regulating cell proliferation, differentiation, and survival. ERK 5 activation requires dual phosphorylation at specific residues, which is essential for enzymatic activity and subsequent phosphorylation of downstream targets. ERK 5 localizes in the nucleus, where ERK 5 influences gene expression and cellular responses to external stimuli, making ERK 5 a vital component in the regulation of various physiological processes. ERK 5 interaction with other MAP kinases, such as ERK 1 and ERK 2, underscores the complexity of signaling networks that govern cellular behavior, highlighting the significance of anti-ERK 5 antibody (1.T218/Y220) in studying these pathways.

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p-ERK 5 Antibody (1.T218/Y220) References:

1. ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ.  |  Haycock, JW., et al. 1992. Proc Natl Acad Sci U S A. 89: 2365-9. PMID: 1347949
2. Purification of a murine protein-tyrosine/threonine kinase that phosphorylates and activates the Erk-1 gene product: relationship to the fission yeast byr1 gene product.  |  Crews, CM. and Erikson, RL. 1992. Proc Natl Acad Sci U S A. 89: 8205-9. PMID: 1381507
3. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
4. LIGHT induces differentiation of mouse embryonic stem cells associated with activation of ERK5.  |  Zou, GM., et al. 2006. Oncogene. 25: 463-9. PMID: 16247486
5. Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase.  |  Boulton, TG., et al. 1991. Biochemistry. 30: 278-86. PMID: 1846291
6. Identification of the regulatory phosphorylation sites in pp42/mitogen-activated protein kinase (MAP kinase).  |  Payne, DM., et al. 1991. EMBO J. 10: 885-92. PMID: 1849075
7. MEK5 and ERK5 are mediators of the pro-myogenic actions of IGF-2.  |  Carter, EJ., et al. 2009. J Cell Sci. 122: 3104-12. PMID: 19654213
8. Neurotrophin-3 provides neuroprotection via TrkC receptor dependent pErk5 activation in a rat surgical brain injury model.  |  Akyol, O., et al. 2018. Exp Neurol. 307: 82-89. PMID: 29883578
9. Proline-rich extensin-like receptor kinases PERK5 and PERK12 are involved in pollen tube growth.  |  Borassi, C., et al. 2021. FEBS Lett. 595: 2593-2607. PMID: 34427925
10. Components of a new human protein kinase signal transduction pathway.  |  Zhou, G., et al. 1995. J Biol Chem. 270: 12665-9. PMID: 7759517
11. ERK6, a mitogen-activated protein kinase involved in C2C12 myoblast differentiation.  |  Lechner, C., et al. 1996. Proc Natl Acad Sci U S A. 93: 4355-9. PMID: 8633070

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-ERK 5 Antibody (1.T218/Y220) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-p-ERK 5 Antibody (1.T218/Y220) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-ERK 5 Antibody (1.T218/Y220) Specifications

Alternate Product Name: ERK 5

Product Clone Name: 1.T218/Y220

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Thr 218/Tyr 220

Epitope Target: ERK 5

Epitope Species: human

Product Reactivity/Detection: Thr 218 and Tyr 220 dually phosphorylated ERK 5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for p-ERK 5 Antibody (1.T218/Y220): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin