Date published: 2025-9-8

00800 4573 8000

SCBT Portrait Logo
Seach Input

COL1A2 Anticorpo (E-6): sc-393573

4.6(16)
Escrever uma avaliaçãoFazer uma pergunta

Fichas de dados
  • COL1A2 Anticorpo E-6é um anticorpo monoclonal produzido em camundongo IgG1 λ COL1A2 anticorpo, citado em 19 publicações, fornecido em 200 µg/ml
  • específico para um epítopo mapeado entre os aminoácidos1064-1093 no C-terminus de Collagen α2 Type I de mouse origem
  • COL1A2 Anticorpo (E-6) é recomendado para a detecção de Collagen α2 Type I de mouse, rat e human origem em WB, IP, IF, IHC(P) e ELISA
  • Anticorpo anti-COL1A2 O anticorpo anti-COL1A2 (E-6) está disponível conjugado com agarose para IP; HRP para WB, IHC(P) e ELISA; e com fioeritrina ou FITC para IF, IHC(P) e FCM
  • também disponível conjugado com Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 para WB (RGB), IF, IHC(P) e FCM, e para utilização com sistemas de imagiologia fluorescente RGB, tais como iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • também disponível conjugado com Alexa Fluor® 680 ou Alexa Fluor® 790 para WB (NIR), IF e FCM; para utilização com sistemas de deteção de infravermelhos próximos (NIR), tais como LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure e outros sistemas comparáveis
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de COL1A2 (E-6): sc-393573.
  • m-IgGλ BP-HRP (camundongo IgGλ ligação protéica-HRP) é o reagente de deteção secundária preferido para COL1A2 Antibody (E-6) para aplicações WB and IHC(P). Este reagente é agora oferecido num pacote com o anticorpo COL1A2 (E-6) (ver informações para encomenda abaixo). Para mais conjugados m-IgGλ BP, ver a nossa lista completa de Proteínas de ligação protéica à IgG do camundongo.

LINKS RÁPIDOS

VEJA TAMBÉM

O anticorpo COL1A2 (E-6) é um anticorpo monoclonal COL1A2 de camundongo IgG1 λ (também designado anticorpo específico de produto do gene col1a2 ou anticorpo de cadeia alfa 2 de colágeno tipo I (COL1A2)) que detecta a proteína COL1A2 de origem de camundongo, rato e humana por WB, IP, IF, IHC(P) e ELISA. O anticorpo COL1A2 (E-6) está disponível na forma de anticorpo anti-COL1A2 não conjugado, bem como em várias formas conjugadas de anticorpo anti-COL1A2, incluindo agarose, HRP, PE, FITC e vários conjugados Alexa Fluor®. A extensa família de produtos do gene COL (colágenos) é composta por vários tipos de cadeia, incluindo colágenos intersticiais formadores de fibrilas (tipos I, II, III e V) e colágenos de membrana basal (tipo IV), cada tipo contendo várias isoformas. Os colágenos são proteínas fibrosas da matriz extracelular com alta resistência à tração e são os principais componentes do tecido conjuntivo, como tendões e cartilagem. Todos os colágenos contêm um domínio de hélice tripla e frequentemente apresentam autoassociação lateral para formar tecidos conjuntivos complexos. Vários colágenos também desempenham um papel na adesão celular, importante para manter a arquitetura e a função normais do tecido.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

LI-COR® e Odyssey® são marcas registadas da LI-COR Biosciences

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

COL1A2 Anticorpo (E-6)

sc-393573
200 µg/ml
$316.00

Pacote do COL1A2 (E-6): m-IgGλ BP-HRP

sc-524005
200 µg Ab, 40 µg BP
$354.00

COL1A2 Anticorpo (E-6) AC

sc-393573 AC
500 µg/ml, 25% agarose
$416.00

COL1A2 Anticorpo (E-6) HRP

sc-393573 HRP
200 µg/ml
$316.00

COL1A2 Anticorpo (E-6) FITC

sc-393573 FITC
200 µg/ml
$330.00

COL1A2 Anticorpo (E-6) PE

sc-393573 PE
200 µg/ml
$343.00

COL1A2 Anticorpo (E-6) Alexa Fluor® 488

sc-393573 AF488
200 µg/ml
$357.00

COL1A2 Anticorpo (E-6) Alexa Fluor® 546

sc-393573 AF546
200 µg/ml
$357.00

COL1A2 Anticorpo (E-6) Alexa Fluor® 594

sc-393573 AF594
200 µg/ml
$357.00

COL1A2 Anticorpo (E-6) Alexa Fluor® 647

sc-393573 AF647
200 µg/ml
$357.00

COL1A2 Anticorpo (E-6) Alexa Fluor® 680

sc-393573 AF680
200 µg/ml
$357.00

COL1A2 Anticorpo (E-6) Alexa Fluor® 790

sc-393573 AF790
200 µg/ml
$357.00

COL1A2 (E-6) peptídeo neutralizante

sc-393573 P
100 µg/0.5 ml
$68.00

您好,我之前问的问题不是能不能透膜,您回答的不是我的问题,我问的问题是拿荧光标记的这个抗体做流式的时候,是否需要透膜?这个抗体结合的部位是细胞内还是细胞外?谢谢。Do the cells need to be permeabilized for the antibody to bind?

Asked by: dxl3742
感谢提问。 目前我们所掌握的信息,正常情况下这个蛋白定位是细胞外,谢谢。 Thank you for your question. The protein is located extracellular.
Answered by: Ling Chen
Date published: 2022-07-11

Hello, for flow cytometry is this stained directly or do the cells need to be permeabilized?

Asked by: dxl3742
Thank you for your question. If the experiment requires us to do cell permeability, we will do. 感谢提问。 如果实验需要,我们可以进行细胞通透。
Answered by: Ling Chen
Date published: 2022-07-07

マウスの組織で、WBとIFあるいはIHCを考えています。 CollagenⅠ A1との交差反応が見られるのでしょうか。 あるいはA1は染まらず、A2のみ特異的に検出できるのでしょうか。

Asked by: Tom0112
Thank you for your question. Please contact our Technical Service Department and we will be happy to assist you. You may call us directly at 001 800-1338-3838, email at asia@scbio.cn, or contact us by live chat. お問い合わせを頂きまして、誠にありがとうございました。お手数ですが、電話 001 800-1338-3838、メールasia@scbio.cnまたはライブチャットで、当社のテクニカルサービスまでお問い合わせください。その他の不明点等がございましたら、またご連絡ください。
Answered by: Technical Support 8
Date published: 2018-12-21

To my question about IHC", you mentioned the product COL1A2 (E-6): sc-393573. But the description of the product suggest "HRP (sc-393573 HRP)" for IHC(P). Is there no difference between the products "sc-393573" and "sc-393573 HRP" for IHC(P) and WB?

Asked by: David27
Thank you for your question. The difference between the catalog numbers, sc-393573 and sc-393573 HRP, is that the latter is the primary antibody directly conjugated to horse radish peroxidase (HRP). This antibody is suitable for WB and IHC(P), and if you purchase the unconjugated antibody, you will need to use a secondary antibody, or mouse IgG binding protein for detection. Alternatively, if you prefer to use a directly conjugated primary antibody, you can purchase the HRP conjugated antibody and also use it for both WB and IHC(P).
Answered by: Technical Support
Date published: 2023-09-23

Also, what is the recommended secondary antibody in IHC? There are only those written for IF, WB etc

Asked by: David27
Thank you for your question. We would recommend using our HRP-conjugated mouse IgG binding protein, m-IgGκ BP-HRP (sc-516102), for indirect immunoperoxidase staining, in lieu of conventional anti-mouse secondary antibodies. Alternatively, we would recommend if you are using the Avidin-Biotin Complex (ABC) staining protocol to select any conventional biotinylated anti-mouse IgG secondary antibody and use in conjunction with our ImmunoCruz® ABC Kit (sc-516216).
Answered by: Technical Support
Date published: 2017-07-20

What is the recommended agent for antigen unmasking in IHC

Asked by: David27
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval methods using this antibody, COL1A2 (E-6): sc-393573. A link to our recommended protocol is below. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-07-20

What application is the blocking peptide sc-393573 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 15
Date published: 2023-10-21
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_7
  • loc_pt_PT, sid_393573, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 137ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from This antibody works very well for IFC and IHQThis antibody works very well for immunofluorescence and immunhistochemistry (mouse lung tissue)
Date published: 2023-06-20
Rated 3 out of 5 by from Tested on HFFF by FACSThis antibody was tested on HFFF cells by FACS and gave a satisfactory signal at dilution 1:25
Date published: 2018-11-19
Rated 4 out of 5 by from COL1A2 Antibody (E-6)COL1A2 (E-6): sc. Near-infrared western blot analysis of LDH-A expression in MEF whole cell lysates. Blocked with 5% NFDM for 1 hour. Detection reagent used: DAM CW800 (Licor).
Date published: 2018-06-28
Rated 5 out of 5 by from 抗体很不错抗体很好用,特异性好,就是效价低了点,其他都不错,Santa的抗体就是很好用,不错的选择,还送优惠券
Date published: 2018-04-28
Rated 5 out of 5 by from great antibodyIt is great for western blot and staining for mouse heart sample.
Date published: 2017-12-14
Rated 4 out of 5 by from Good antibody for IFI Used 1:200 dilution in paraffined mouse kidney. I have got good IF photos.
Date published: 2017-08-28
Rated 4 out of 5 by from good for WBI use 1:500 dilution and get clear band at 130kd, but have some undesired bands
Date published: 2017-08-18
Rated 5 out of 5 by from The antibody is goodThis antibody is very sutiable for WB, it shows a very clear band.
Date published: 2017-07-25
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_16
  • loc_pt_PT, sid_393573, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT
COL1A2 Anticorpo (E-6) is rated 4.6 out of 5 by 16.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_16
  • loc_pt_PT, sid_393573, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 114ms
  • REVIEWS, PRODUCT