Date published: 2025-9-8

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cathepsin D Anticorpo (D-7): sc-377299

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Fichas de dados
  • cathepsin D Anticorpo D-7é um anticorpo monoclonal produzido em camundongo IgM κ cathepsin D anticorpo, citado em 49 publicações, fornecido em 200 µg/ml
  • específico para um epítopo mapeado entre os aminoácidos383-411 no C-terminus de cathepsin D de human origem
  • recomendado para a detecção de cathepsin D de mouse, rat e human origem em
  • disponível conjugado à agarose/b> para IP; HRP/b> para WB, IHC(P) e ELISA; ficoeritrina/b>, FITC/b>, Alexa Fluor® 488/b> ou Alexa Flúor® 647/b> para IF, IHC(P) e FCM
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de cathepsin D (D-7): sc-377299.
  • m-IgGκ BP-HRP é o reagente de deteção secundária preferido para cathepsin D Anticorpo (D-7) para aplicações WB and IHC(P). Este reagente é agora oferecido num pacote com o anticorpo cathepsin D (D-7) (ver informações para encomenda abaixo). Para mais conjugados m-IgGκ BP, ver a nossa lista completa de Proteínas de ligação à IgG do camundongo.

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VEJA TAMBÉM

O anticorpo contra catepsina D (D-7) é um anticorpo monoclonal IgM κ de camundongo contra catepsina D (também designado anticorpo CTSD) que detecta a proteína catepsina D de origem de camundongo, rato e humana por WB, IP, IF, IHC(P) e ELISA. O anticorpo catepsina D (D-7) está disponível na forma de anticorpo anti-catepsina D não conjugado, bem como em várias formas conjugadas de anticorpo anti-catepsina D, incluindo agarose, HRP, PE, FITC e vários conjugados Alexa Fluor®. A família de enzimas proteolíticas da catepsina contém diversas classes de proteases. A classe da cisteína protease compreende as catepsinas B, L, H, K, S e O. A classe da aspartil protease é composta pelas catepsinas D e E. A catepsina G pertence à classe da serina protease. A maioria das catepsinas é lisossômica e cada uma delas está envolvida no metabolismo celular, participando de vários eventos, como a biossíntese de peptídeos e a degradação de proteínas. As catepsinas também podem dividir alguns precursores de proteínas, liberando assim peptídeos reguladores. A região promotora do gene da catepsina D contém cinco sítios de ligação Sp1 e quatro sítios de ligação AP-2.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Alexa Fluor® é uma marca comercial da Molecular Probes Inc., OR., EUA

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

cathepsin D Anticorpo (D-7)

sc-377299
200 µg/ml
$316.00

Pacote do cathepsin D (D-7): m-IgGκ BP-HRP

sc-550757
200 µg Ab; 40 µg BP
$354.00

cathepsin D Anticorpo (D-7) AC

sc-377299 AC
500 µg/ml, 25% agarose
$416.00

cathepsin D Anticorpo (D-7) Alexa Fluor® 488

sc-377299 AF488
200 µg/ml
$357.00

cathepsin D Anticorpo (D-7) Alexa Fluor® 647

sc-377299 AF647
200 µg/ml
$357.00

cathepsin D Anticorpo (D-7) FITC

sc-377299 FITC
200 µg/ml
$330.00

cathepsin D Anticorpo (D-7) HRP

sc-377299 HRP
200 µg/ml
$316.00

cathepsin D (D-7) peptídeo neutralizante

sc-377299 P
100 µg/0.5 ml
$68.00

cathepsin D Anticorpo (D-7) PE

sc-377299 PE
200 µg/ml
$343.00

What is recommendable for a WesternBlot, using cathepsin D Antibody (D-7): sc-377299 and a secondary antibody or use directly cathepsin D Antibody (D-7): sc-377299 conjugated with HRP? Thanks

Asked by: CUES
Thank you for your question. It is by personal preference to reduce the number of steps on your protocol we recommend using the HRP conjugated form. https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: BlakeJ
Date published: 2023-08-18

Does it work in pig tissue? Thanks.

Asked by: YMC39
Thank you for your question. We validate this antibody for mouse, rat and human. There is a 78% homology in pig. We only honor our warranty if the homology is 95% or higher.
Answered by: BlakeJ
Date published: 2023-03-30

Does this work by immunofluorescence in mouse cells? Is there a recommended dilution for this application? Thank you.

Asked by: scienceguy
Anti-cathepsin D Antibody (D-7): sc-377299 is recommended for detection of cathepsin D of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. For immunofluorescence, the recommended starting dilution is 1:50, with a dilution range between 1:50-1:500.
Answered by: Tech Support Europe
Date published: 2021-08-25

I will use this antibody (sc-377299) in western blot. Can you suggest second antibody (HRP conjugated) for me? Thanks.

Asked by: Researcherman
Thank you for your question. As secondary antibody with HRP conjugate we recommend m-IgGκ BP-HRP: sc-516102
Answered by: Tech Support Europe
Date published: 2021-07-14

What is the best reagent to dilute this antibody for Western Blotting?

Asked by: MZOIA
Thank you for your question. We recommend using our TBS Blotto A: sc-2333 (https://www.scbt.com/scbt/product/tbs-blotto-a) which is 5% nonfat milk + 1X TBS + 0.05% Tween-20. This is the recommended buffer for blocking, as well as primary and secondary antibody incubations for Western blotting.
Answered by: Technical Support
Date published: 2017-11-08

cathepsin D (D-7): sc-377299 is a mouse IgM, can I use an anti-mouse IgG secondary antibody for detection?

Asked by: cjMara
We recommend using an isotype-specific anti-mouse IgM secondary antibody for detection of this mouse IgM primary antibody. Anti-mouse IgG secondary antibodies may work, due to the similar structures of IgG and IgM, but they are not ideally suited.
Answered by: Technical Support
Date published: 2017-02-02
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Rated 3 out of 5 by from This antibosy works with western blottingThis antibody works with western blotting with SAS cells lysate.
Date published: 2020-06-12
Rated 4 out of 5 by from It works wellWe checked this antibody on lysates from immortalized human proximal tubular epithelial cells by Western blot. It detects a single band (around 48-50 kDa, presumably the immature form) with no background signals, after overnight incubation (1:500) at 4°C. We were unable to detect the mature form of Cathepsin D (35 kDa) in these cells upon these experimental conditions.
Date published: 2019-06-12
Rated 5 out of 5 by from works very well for mouse fat tissueI did 1/500 dilution for 4 degree overnight incubation, which gives me expected band, upper band between 40-55 kDa, around 52Kda or 46 kDa, I am not sure. But it also can detect the mouse IgG light chain, the bottom band
Date published: 2017-12-21
Rated 3 out of 5 by from Working antibody in WBThis antibody works ok in WB with human and mouse samples. There are quite a few non-specific bands.
Date published: 2017-10-18
Rated 5 out of 5 by from it's excitingWe made an experiment with cathepsin D antibody and found it very useful. This antibody has very good activity and a demonstrable effect.
Date published: 2017-10-18
Rated 1 out of 5 by from It doesn't work in IF in rat 832/13 ins1 cellsPFA fixed, permeabilized with Triton-X10. Used at 1:50 for IF
Date published: 2017-10-15
Rated 5 out of 5 by from good featuresuse in NB4 cells ,the trips is very abvious,thank you for your free sample
Date published: 2017-09-06
Rated 5 out of 5 by from Works greatWorks great with no background signals on western blot at 1:200 in mouse cell line
Date published: 2017-08-03
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cathepsin D Anticorpo (D-7) is rated 4.4 out of 5 by 20.
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