ZWINT Antibody (G-12): sc-166730
- ZWINT Antibody (G-12) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-277 deletion 228-229 (deletion 228-229) representing full length ZWINT of human origin
- ZWINT Antibody (G-12) is recommended for detection of ZWINT of human origin by WB, IP, IF and ELISA
- Anti-ZWINT Antibody (G-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for ZWINT Antibody (G-12) for WB applications. These reagents are now offered in bundles with ZWINT Antibody (G-12) (see ordering information below).
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SEE ALSO...
ZWINT Antibody (G-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects ZWINT of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ZWINT Antibody (G-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZWINT, also known as KNTC2AP or HZwint-1, is a 277 amino acid protein crucial for proper kinetochore function, which is essential for accurate chromosome segregation during cell division. ZWINT is localized to the cytoplasm during interphase and translocates to kinetochores from late prophase to anaphase, where ZWINT plays a pivotal role in regulating the interaction between ZW10 (Zeste White 10) and kinetochores. ZWINT is a key component of a kinetochore complex that includes proteins such as MIS12 (MIND kinetochore complex component) and PMF-1 (polyamine-modulated factor 1), which collectively ensure the integrity of kinetochore formation and the spindle checkpoint mechanism. Disruptions in the gene encoding ZWINT have been linked to Roberts syndrome, an autosomal recessive disorder characterized by growth retardation due to premature chromosome separation, highlighting ZWINT′s importance in maintaining genomic stability.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ZWINT Antibody (G-12) References:
- HZwint-1, a novel human kinetochore component that interacts with HZW10. | Starr, DA., et al. 2000. J Cell Sci. 113 (Pt 11): 1939-50. PMID: 10806105
- Implication of ZW10 in membrane trafficking between the endoplasmic reticulum and Golgi. | Hirose, H., et al. 2004. EMBO J. 23: 1267-78. PMID: 15029241
- Recapitulation of the Roberts syndrome cellular phenotype by inhibition of INCENP, ZWINT-1 and ZW10 genes. | Musio, A., et al. 2004. Gene. 331: 33-40. PMID: 15094189
- Human Zwint-1 specifies localization of Zeste White 10 to kinetochores and is essential for mitotic checkpoint signaling. | Wang, H., et al. 2004. J Biol Chem. 279: 54590-8. PMID: 15485811
- A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1. | Obuse, C., et al. 2004. Nat Cell Biol. 6: 1135-41. PMID: 15502821
- ZW10 links mitotic checkpoint signaling to the structural kinetochore. | Kops, GJ., et al. 2005. J Cell Biol. 169: 49-60. PMID: 15824131
- Measuring the stoichiometry and physical interactions between components elucidates the architecture of the vertebrate kinetochore. | Emanuele, MJ., et al. 2005. Mol Biol Cell. 16: 4882-92. PMID: 16079178
- Hec1 sequentially recruits Zwint-1 and ZW10 to kinetochores for faithful chromosome segregation and spindle checkpoint control. | Lin, YT., et al. 2006. Oncogene. 25: 6901-14. PMID: 16732327
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZWINT Antibody (G-12) | sc-166730 | 200 µg/ml | $322.00 | |||
ZWINT Antibody (G-12): m-IgG Fc BP-HRP Bundle | sc-527179 | 200 µg Ab; 10 µg BP | $361.00 | |||
ZWINT Antibody (G-12): m-IgG1 BP-HRP Bundle | sc-532552 | 200 µg Ab; 20 µg BP | $361.00 | |||
ZWINT Antibody (G-12) AC | sc-166730 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ZWINT Antibody (G-12) HRP | sc-166730 HRP | 200 µg/ml | $322.00 | |||
ZWINT Antibody (G-12) FITC | sc-166730 FITC | 200 µg/ml | $336.00 | |||
ZWINT Antibody (G-12) PE | sc-166730 PE | 200 µg/ml | $349.00 | |||
ZWINT Antibody (G-12) Alexa Fluor® 488 | sc-166730 AF488 | 200 µg/ml | $364.00 | |||
ZWINT Antibody (G-12) Alexa Fluor® 546 | sc-166730 AF546 | 200 µg/ml | $364.00 | |||
ZWINT Antibody (G-12) Alexa Fluor® 594 | sc-166730 AF594 | 200 µg/ml | $364.00 | |||
ZWINT Antibody (G-12) Alexa Fluor® 647 | sc-166730 AF647 | 200 µg/ml | $364.00 | |||
ZWINT Antibody (G-12) Alexa Fluor® 680 | sc-166730 AF680 | 200 µg/ml | $364.00 | |||
ZWINT Antibody (G-12) Alexa Fluor® 790 | sc-166730 AF790 | 200 µg/ml | $364.00 |