Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ZNF703 CRISPR/Cas9 KO Plasmid (h): sc-406411
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZNF703 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZNF703 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ZNF703 Antibody (E-6): sc-271896
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZNF703 CRISPR/Cas9 KO Plasmid (h) | sc-406411 | 20 µg | $397.00 |
Overview
ZNF703 (zinc finger protein 703) is a nuclear transcriptional regulator implicated in controlling gene expression programs linked to epithelial differentiation, cell-cycle progression, and lineage specification. As a member of the NET/NLZ family, ZNF703 can function within transcriptional repression and chromatin-associated complexes to modulate developmental signaling and downstream transcriptional networks. Dysregulated ZNF703 expression has been associated with altered proliferative and migratory phenotypes and is frequently studied in the context of 8p12 amplicon biology in breast cancer research. These features make ZNF703 a useful target for investigating transcription factor–driven regulatory circuitry, oncogenic transcriptional states, and pathway dependencies in human cell models.
ZNF703 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZNF703 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZNF703 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZNF703 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZNF703 protein expression.
This CRISPR knockout system enables efficient generation of ZNF703-deficient cell models for investigation of ZNF703 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZNF703 exon(s) critical for ZNF703 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZNF703 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZNF703 CRISPR/Cas9 KO Plasmid (h) and ZNF703 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZNF703 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZNF703 HDR Plasmid (h) and ZNF703 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZNF703 homology arms to support homology-directed repair at defined ZNF703 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.