Cre Vector Cre Vector is designed to specifically target the LoxP sites flanking selection markers inserted by the HDR Plasmid and excise the genetic material

Cre Vector

Cre Vector is rated 4.5 out of 5 by 2.
  • y_2020, m_2, d_20, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.5
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_418923, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 9ms
  • REVIEWS, PRODUCT
5
1
4
1
3
0
2
0
1
0

Datasheets

    • 20 µg of transfection-ready, purified plasma DNA; Suitable for up to 20 transfections
    • Cre Vector expresses Cre recombinase to catalyze site-specific DNA recombination between two LoxP sites
    • Cre Vector is designed to specifically target the LoxP sites flanking selection markers inserted by the HDR Plasmid and excise the genetic material
    • Cre Vector contains a CMV promoter to drive expression of Cre recombinase
    • Following transfection, cells can be assayed by WB, IF or IHC using antibody for target gene, or re-transfected for additional DNA editing
Submit a review for this product and receive 15 CruzCredits

Hi, can the above plasmid be used for yeast namely saccharomyces cerevisiae?

Asked by: NT777
Thank you for your question. No, our Cre vector is specific for use with our knockout and HDR CRISPR reagents, and can only be used on mammalian cells.
Answered by: Tech Service
Date published: 2020-02-10

whats the cre loxp systems

Asked by: waleed xel
Thank you for your question. The Cre-Lox system is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. You can find our more about the Cre-Lox system at any of the links below: https://en.wikipedia.org/wiki/Cre-Lox_recombination https://www.youtube.com/watch?v=oLPjiwM0G7A&app=desktop
Answered by: Tech Service
Date published: 2019-04-09

Which antibiotic gene does it have?

Asked by: Sev05
Thank you for your question. The Cre vector does not have a selection gene. It is only used to remove the puromycin resistance following an HDR knockdown experiment. You can read more about how it works here: https://www.scbt.com/scbt/whats-new/crispr-systems
Answered by: Tech Service
Date published: 2019-02-14

Does it have amp+ marker?

Asked by: Jit98
Thank you for your question. This product is provided ready to use, and does not need amplification. You can see components of the plasmid and how it works here: https://www.scbt.com/scbt/whats-new/crispr-systems
Answered by: Technical Support
Date published: 2019-01-26
  • y_2020, m_2, d_20, h_15CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.5
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_en_US, sid_418923, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 22ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Very useful product. Very useful tool to achieve double or triple knock-out with the CRISPR system since the most of Santa Cruz CRISPR products are resistant to the same antibiotic-pyromycin.
Date published: 2017-04-25
Rated 4 out of 5 by from Remove the marker gene efficiently I used this vector to remove the neo marker-gene in the pig primary cells . The marker-free efficiency was so high to 80% .I nucleofected the 4ug Cre vector to the 1000000 cells by ALONZO. But, i suggested that can the CAG promoter may replace the CMV promoter of this vector, because of the CMV promoter may Cause gene-silencing in some special cell lines
Date published: 2016-11-21
  • y_2020, m_2, d_20, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.5
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_418923, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.