Cre Vector Cre Vector is designed to specifically target the LoxP sites flanking selection markers inserted by the HDR Plasmid and excise the genetic material

Cre Vector

Cre Vector is rated 4.5 out of 5 by 2.
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Datasheets

    • 20 µg of transfection-ready, purified plasma DNA; Suitable for up to 20 transfections
    • Cre Vector expresses Cre recombinase to catalyze site-specific DNA recombination between two LoxP sites
    • Cre Vector is designed to specifically target the LoxP sites flanking selection markers inserted by the HDR Plasmid and excise the genetic material
    • Cre Vector contains a CMV promoter to drive expression of Cre recombinase
    • Following transfection, cells can be assayed by WB, IF or IHC using antibody for target gene, or re-transfected for additional DNA editing

How large is this plasmid?

Asked by: Dkr0
Thank you for your question. Our Cre Vector sc-418923 has a size of approximately 5.9 kilobases.
Answered by: Tech Support Europe
Date published: 2020-07-09

Why is CRE Vector Transfection "Recommended" after for CRISPR/Cas9 KO Plasmid and HDR Plasmid Transfection (following selection)? Is it simply to remove the selection marker? Or, does it help in any way with knocking out the gene?

Asked by: jmd6207
Thank you for your question. It is not mandatory, and only recommended if you wish to removed the puromycin selection marker. Removing the HDR plasmid insert using the CRE vector does not help with knocking out the gene. It is useful if you wish you knock out a second gene or if you need you cells to no longer be puromycin resistant.
Answered by: Tech Service
Date published: 2020-06-02

How to transfect the cre-vector to the cells that already have puromycin gene incorporated by HDR vector? transfect, then wait two or three days and consider those cells puromycin free? correct? do I select?

Asked by: michaela
Thank you for your question. Yes, you are correct. The Cre Vector can be transfected following our standard "Phase I" transfection protocol. Following transfection, cells that have been successfully transfected will not have any selection marker because it will be excised from the genome by the Cre recombinase. To confirm this, you can isolate colonies of cells for testing, and expose them to puromycin to confirm that no cells are still expression puromycin resistance.
Answered by: Technical Support
Date published: 2020-04-24

Hi, can the above plasmid be used for yeast namely saccharomyces cerevisiae?

Asked by: NT777
Thank you for your question. No, our Cre vector is specific for use with our knockout and HDR CRISPR reagents, and can only be used on mammalian cells.
Answered by: Tech Service
Date published: 2020-02-10

whats the cre loxp systems

Asked by: waleed xel
Thank you for your question. The Cre-Lox system is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. You can find our more about the Cre-Lox system at any of the links below: https://en.wikipedia.org/wiki/Cre-Lox_recombination https://www.youtube.com/watch?v=oLPjiwM0G7A&app=desktop
Answered by: Tech Service
Date published: 2019-04-09

Which antibiotic gene does it have?

Asked by: Sev05
Thank you for your question. The Cre vector does not have a selection gene. It is only used to remove the puromycin resistance following an HDR knockdown experiment. You can read more about how it works here: https://www.scbt.com/scbt/whats-new/crispr-systems
Answered by: Tech Service
Date published: 2019-02-14

Does it have amp+ marker?

Asked by: Jit98
Thank you for your question. This product is provided ready to use, and does not need amplification. You can see components of the plasmid and how it works here: https://www.scbt.com/scbt/whats-new/crispr-systems
Answered by: Technical Support
Date published: 2019-01-26
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Rated 5 out of 5 by from Very useful product. Very useful tool to achieve double or triple knock-out with the CRISPR system since the most of Santa Cruz CRISPR products are resistant to the same antibiotic-pyromycin.
Date published: 2017-04-25
Rated 4 out of 5 by from Remove the marker gene efficiently I used this vector to remove the neo marker-gene in the pig primary cells . The marker-free efficiency was so high to 80% .I nucleofected the 4ug Cre vector to the 1000000 cells by ALONZO. But, i suggested that can the CAG promoter may replace the CMV promoter of this vector, because of the CMV promoter may Cause gene-silencing in some special cell lines
Date published: 2016-11-21
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