ZNF395 Antibody (C-1): sc-515519
- ZNF395 Antibody (C-1) is a mouse monoclonal IgG1 κ ZNF395 antibody provided at 200 µg/ml
- raised against amino acids 179-268 mapping within an internal region of ZNF395 of human origin
- ZNF395 Antibody (C-1) is recommended for detection of ZNF395 of human origin by WB, IP, IF and ELISA
- Anti-ZNF395 Antibody (C-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ZNF395 Antibody (C-1) for WB applications. These reagents are now offered in bundles with ZNF395 Antibody (C-1) (see ordering information below).
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ZNF395 Antibody (C-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects ZNF395 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ZNF395 (C-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZNF395, also known as Zinc finger protein 395, PBF (Papillomavirus-binding factor), and HDBP2 (Huntington disease gene regulatory region-binding protein 2), is a 513 amino acid protein that plays a crucial role in gene regulation. As a member of the Krüppel C2H2-type zinc-finger protein family, ZNF395 contains a single C2H2-type zinc finger domain, which is essential for binding specific DNA sequences. This binding capability is particularly important as ZNF395 interacts with the 3′-CCGG-5′ sequence within the papillomavirus promoter, adjacent to a RUNX1-binding motif, thereby influencing transcriptional activity. Additionally, ZNF395 has been shown to bind to a critical seven base pair region within the promoter of the Huntington′s disease gene, which is vital for the expression of this gene associated with neurodegeneration. The widespread expression of ZNF395 suggests a shuttling function between the nucleus and cytoplasm, further underscoring potential regulatory roles in various cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ZNF395 Antibody (C-1) References:
- A new cellular factor recognizes E2 binding sites of papillomaviruses which mediate transcriptional repression by E2. | Boeckle, S., et al. 2002. Virology. 293: 103-17. PMID: 11853404
- Novel nuclear shuttle proteins, HDBP1 and HDBP2, bind to neuronal cell-specific cis-regulatory element in the promoter for the human Huntington's disease gene. | Tanaka, K., et al. 2004. J Biol Chem. 279: 7275-86. PMID: 14625278
- Combined human papillomavirus DNA and human papillomavirus-like particle serologic assay to identify women at risk for high-grade cervical intraepithelial neoplasia. | Einstein, MH., et al. 2007. Int J Cancer. 120: 55-9. PMID: 17036320
- Papillomavirus binding factor (PBF)-mediated inhibition of cell growth is regulated by 14-3-3beta. | Sichtig, N., et al. 2007. Arch Biochem Biophys. 464: 90-9. PMID: 17531190
- Papillomavirus binding factor binds to SAP30 and represses transcription via recruitment of the HDAC1 co-repressor complex. | Sichtig, N., et al. 2007. Arch Biochem Biophys. 467: 67-75. PMID: 17897615
- Prognostic impact and immunogenicity of a novel osteosarcoma antigen, papillomavirus binding factor, in patients with osteosarcoma. | Tsukahara, T., et al. 2008. Cancer Sci. 99: 368-75. PMID: 18271936
- Scythe/BAT3 regulates apoptotic cell death induced by papillomavirus binding factor in human osteosarcoma. | Tsukahara, T., et al. 2009. Cancer Sci. 100: 47-53. PMID: 19018758
- Modulation of angiogenic and inflammatory response in glioblastoma by hypoxia. | Murat, A., et al. 2009. PLoS One. 4: e5947. PMID: 19536297
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZNF395 Antibody (C-1) | sc-515519 | 200 µg/ml | $316.00 | |||
ZNF395 Antibody (C-1): m-IgG Fc BP-HRP Bundle | sc-531399 | 200 µg Ab; 10 µg BP | $354.00 | |||
ZNF395 Antibody (C-1): m-IgGκ BP-HRP Bundle | sc-525080 | 200 µg Ab, 40 µg BP | $354.00 | |||
ZNF395 Antibody (C-1): m-IgG1 BP-HRP Bundle | sc-544530 | 200 µg Ab; 20 µg BP | $354.00 | |||
ZNF395 Antibody (C-1) AC | sc-515519 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ZNF395 Antibody (C-1) HRP | sc-515519 HRP | 200 µg/ml | $316.00 | |||
ZNF395 Antibody (C-1) FITC | sc-515519 FITC | 200 µg/ml | $330.00 | |||
ZNF395 Antibody (C-1) PE | sc-515519 PE | 200 µg/ml | $343.00 | |||
ZNF395 Antibody (C-1) Alexa Fluor® 488 | sc-515519 AF488 | 200 µg/ml | $357.00 | |||
ZNF395 Antibody (C-1) Alexa Fluor® 546 | sc-515519 AF546 | 200 µg/ml | $357.00 | |||
ZNF395 Antibody (C-1) Alexa Fluor® 594 | sc-515519 AF594 | 200 µg/ml | $357.00 | |||
ZNF395 Antibody (C-1) Alexa Fluor® 647 | sc-515519 AF647 | 200 µg/ml | $357.00 | |||
ZNF395 Antibody (C-1) Alexa Fluor® 680 | sc-515519 AF680 | 200 µg/ml | $357.00 | |||
ZNF395 Antibody (C-1) Alexa Fluor® 790 | sc-515519 AF790 | 200 µg/ml | $357.00 |