ZNF295 Antibody (D-8): sc-518254

ZNF295 Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects ZNF295 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ZNF295 monoclonal antibody (D-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZNF295, also known as ZBTB21, is a 1066 amino acid protein that belongs to the Krüppel C2H2-type zinc-finger protein family and is primarily localized in the nucleus, where ZNF295 plays a crucial role in transcriptional regulation. ZNF295 features a BTB (POZ) domain and eight C2H2-type zinc fingers, which are essential for DNA-binding capabilities. ZNF295 is phosphorylated in response to DNA damage, highlighting ZNF295′s involvement in cellular stress responses and maintenance of genomic integrity. ZNF295′s interaction with KAP1, through a KRAB domain, facilitates recruitment of histone-modifying proteins, thereby influencing chromatin remodeling and gene expression. This multifaceted role underscores ZNF295′s importance in regulating transcriptional networks and potential implications in various biological processes and diseases. These properties make anti-ZNF295 antibody (D-8) a valuable resource for exploring regulatory mechanisms in transcription, chromatin remodeling, and cellular stress responses.

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ZNF295 Antibody (D-8) References:

1. Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.  |  Nagase, T., et al. 1999. DNA Res. 6: 337-45. PMID: 10574462
2. The KRAB domain of zinc finger gene ZNF268: a potential transcriptional repressor.  |  Sun, Y., et al. 2003. IUBMB Life. 55: 127-31. PMID: 12822888
3. Conservation, diversification and expansion of C2H2 zinc finger proteins in the Arabidopsis thaliana genome.  |  Englbrecht, CC., et al. 2004. BMC Genomics. 5: 39. PMID: 15236668
4. Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization.  |  Jin, J., et al. 2004. Curr Biol. 14: 1436-50. PMID: 15324660
5. A novel subfamily of zinc finger genes involved in embryonic development.  |  Nakamura, M., et al. 2004. J Cell Biochem. 93: 887-95. PMID: 15449319
6. Novel human BTB/POZ domain-containing zinc finger protein ZNF295 is directly associated with ZFP161.  |  Wang, J., et al. 2005. Biochem Biophys Res Commun. 327: 615-27. PMID: 15629158
7. ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.  |  Matsuoka, S., et al. 2007. Science. 316: 1160-6. PMID: 17525332
8. Spotting the enemy within: Targeted silencing of foreign DNA in mammalian genomes by the Krüppel-associated box zinc finger protein family.  |  Wolf, G., et al. 2015. Mob DNA. 6: 17. PMID: 26435754
9. ZNF295-AS1 inhibits autophagy via the ZNF295-AS1/miR-508-5p/ATG7 axis in AS.  |  Qin, QW., et al. 2020. Eur Rev Med Pharmacol Sci. 24: 7024-7032. PMID: 32633396
10. Elevated expression of sepiapterin reductase, regulator of G protein signaling 1, hypothetical protein CXorf58 homolog, and zinc finger and BTB domain-containing protein 21 isoform X2 is associated with progression of hepatocellular carcinoma.  |  Ranjpour, M., et al. 2021. Protoplasma. 258: 1133-1143. PMID: 33683453
11. Zbtb21 is required for the anterior-posterior patterning of neural tissue in the early Xenopus embryo.  |  Takebayashi-Suzuki, K., et al. 2022. Biochem Biophys Res Commun. 630: 190-197. PMID: 36166855
12. ZBTB21 suppresses CRE-mediated transcription to impair synaptic function in Down syndrome.  |  Qiao, M., et al. 2024. Sci Adv. 10: eadm7373. PMID: 38959316

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ZNF295 Antibody (D-8) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-ZNF295 Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ZNF295 Antibody (D-8) Specifications

Alternate Product Name: ZNF295

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 480-508

Epitope Target: ZNF295

Epitope Species: human

Product Reactivity/Detection: ZNF295

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for ZNF295 Antibody (D-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin