ZBP-89 Antibody (H-7): sc-137171

ZBP-89 Antibody (H-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects ZBP-89 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). ZBP-89 (H-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ZBP-89, also known as BFCOL1, BERF1, and ZNF 148, is a zinc finger transcription factor that plays a crucial role in gene regulation and is universally expressed across different tissues. ZBP-89 is primarily located in the nucleus, where ZBP-89 functions as a transcriptional repressor, particularly for genes involved in cell cycle regulation and differentiation. Nuclear localization of ZBP-89 is vital for modulating the expression of target genes, such as the cyclin-dependent kinase inhibitor p21, which is essential for controlling cell proliferation and promoting apoptosis in response to cellular stress. ZBP-89′s ability to bind to GC-rich DNA elements in the promoters of various genes, including those involved in cancer progression, underscores ZBP-89′s importance in maintaining cellular homeostasis and potential as a therapeutic target in cancer treatment. Elevated levels of ZBP-89 have been associated with growth arrest and apoptosis in human gastrointestinal cells, highlighting ZBP-89′s significance in cancer biology and cell fate regulation.

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ZBP-89 Antibody (H-7) References:

1. The zinc finger repressor, ZBP-89, binds to the silencer element of the human vimentin gene and complexes with the transcriptional activator, Sp1.  |  Wieczorek, E., et al. 2000. J Biol Chem. 275: 12879-88. PMID: 10777586
2. Transcription factor ZBP-89 cooperates with histone acetyltransferase p300 during butyrate activation of p21waf1 transcription in human cells.  |  Bai, L. and Merchant, JL. 2000. J Biol Chem. 275: 30725-33. PMID: 10899165
3. Identification and characterization of a transcriptional regulator for the lck proximal promoter.  |  Yamada, A., et al. 2001. J Biol Chem. 276: 18082-9. PMID: 11278409
4. ZBP-89 promotes growth arrest through stabilization of p53.  |  Bai, L. and Merchant, JL. 2001. Mol Cell Biol. 21: 4670-83. PMID: 11416144
5. Transcription factor ZBP-89 is required for STAT1 constitutive expression.  |  Bai, L. and Merchant, JL. 2003. Nucleic Acids Res. 31: 7264-70. PMID: 14654702
6. ATM phosphorylates ZBP-89 at Ser202 to potentiate p21waf1 induction by butyrate.  |  Bai, L. and Merchant, JL. 2007. Biochem Biophys Res Commun. 359: 817-21. PMID: 17560543
7. Neuronal insulin receptor substrate 2 (IRS2) expression is regulated by ZBP89 and SP1 binding to the IRS2 promoter.  |  Udelhoven, M., et al. 2010. J Endocrinol. 204: 199-208. PMID: 19875459
8. ZBP-89 enhances Bak expression and causes apoptosis in hepatocellular carcinoma cells.  |  To, AK., et al. 2011. Biochim Biophys Acta. 1813: 222-30. PMID: 20850481
9. Promoter cloning and characterization of the human programmed cell death protein 4 (pdcd4) gene: evidence for ZBP-89 and Sp-binding motifs as essential Pdcd4 regulators.  |  Leupold, JH., et al. 2012. Biosci Rep. 32: 281-97. PMID: 22111549
10. Epigenetic upregulation of Bak by ZBP-89 inhibits the growth of hepatocellular carcinoma.  |  Ye, CG., et al. 2013. Biochim Biophys Acta. 1833: 2970-2979. PMID: 23954442
11. Overexpression of ZBP-89, a zinc finger DNA binding protein, in gastric cancer.  |  Taniuchi, T., et al. 1997. Biochem Biophys Res Commun. 233: 154-60. PMID: 9144414
12. ZBP-89, a Krüppel-type zinc finger protein, inhibits cell proliferation.  |  Remington, MC., et al. 1997. Biochem Biophys Res Commun. 237: 230-4. PMID: 9268691

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ZBP-89 Antibody (H-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-ZBP-89 Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ZBP-89 Antibody (H-7) Specifications

Alternate Product Name: ZBP-89

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 611-794

Epitope Target: ZBP-89

Epitope Species: human

Product Reactivity/Detection: ZBP-89

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for ZBP-89 Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent