ZBP-89 Antibody (D-10): sc-137170

ZBP-89 Antibody (D-10) is a mouse monoclonal IgG1 antibody that detects ZBP-89 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ZBP-89, also known as BFCOL1, BERF1, and ZNF 148, is a zinc finger transcription factor that plays a crucial role in regulating gene expression, particularly as a silencer element binding factor for Vimentin. ZBP-89 is universally expressed and functions as a Kruppel-like repressor, binding to GC-rich DNA elements in the promoters of key genes, including gastrin, ornithine decarboxylase, and the cyclin-dependent kinase inhibitor p21, which is a significant target of the tumor suppressor protein p53. ZBP-89′s interaction with p53 is particularly important, as this interaction not only regulates cell proliferation but also influences the cellular response to stress by retarding the nuclear export of p53, thereby enhancing tumor-suppressive functions. ZBP-89 expression can be induced by agents such as trans-retinoic acid or butyrate, which are known to promote terminal differentiation in colon cancer cells, highlighting a potential role in cancer biology. Elevated levels of ZBP-89 have been associated with growth arrest and apoptosis in human gastrointestinal cells, making ZBP-89 a critical factor in understanding cellular mechanisms related to cancer and differentiation.

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ZBP-89 Antibody (D-10) References:

1. ZBP-89 promotes growth arrest through stabilization of p53.  |  Bai, L. and Merchant, JL. 2001. Mol Cell Biol. 21: 4670-83. PMID: 11416144
2. ZBP-89 represses vimentin gene transcription by interacting with the transcriptional activator, Sp1.  |  Zhang, X., et al. 2003. Nucleic Acids Res. 31: 2900-14. PMID: 12771217
3. ZBP-89-induced apoptosis is p53-independent and requires JNK.  |  Bai, L., et al. 2004. Cell Death Differ. 11: 663-73. PMID: 14963412
4. Intestinal alkaline phosphatase gene expression is activated by ZBP-89.  |  Malo, MS., et al. 2006. Am J Physiol Gastrointest Liver Physiol. 290: G737-46. PMID: 16384873
5. An isoform of ZBP-89 predisposes the colon to colitis.  |  Law, DJ., et al. 2006. Nucleic Acids Res. 34: 1342-50. PMID: 16517939
6. The transcription factor ZBP-89 suppresses p16 expression through a histone modification mechanism to affect cell senescence.  |  Feng, Y., et al. 2009. FEBS J. 276: 4197-206. PMID: 19583777
7. Increased expression of ZBP-89 and its prognostic significance in hepatocellular carcinoma.  |  Zhang, CZ., et al. 2012. Histopathology. 60: 1114-24. PMID: 22372401
8. High-expression of ZBP-89 correlates with distal metastasis and poor prognosis of patients in clear cell renal cell carcinoma.  |  Cai, MY., et al. 2012. Biochem Biophys Res Commun. 426: 636-42. PMID: 22982674
9. Zfp281 (ZBP-99) plays a functionally redundant role with Zfp148 (ZBP-89) during erythroid development.  |  Woo, AJ., et al. 2019. Blood Adv. 3: 2499-2511. PMID: 31455666
10. Transcription factor ZBP-89 regulates the activity of the ornithine decarboxylase promoter.  |  Law, GL., et al. 1998. J Biol Chem. 273: 19955-64. PMID: 9685330

The preferred ZBP-89 antibody is ZBP-89 Antibody (H-7): sc-137171.