ZAP-70 Antibody (3D2A4): sc-53899

ZAP-70 Antibody (3D2A4) is a mouse monoclonal IgG2a antibody that detects ZAP-70 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). ZAP-70, a crucial tyrosine kinase, plays a significant role in T cell receptor signaling, which is essential for T lymphocyte activation and subsequent immune responses. Located primarily in the cytoplasm, ZAP-70 associates with the ζ chain of the T cell receptor complex, where ZAP-70 becomes activated following TCR stimulation. This activation leads to the phosphorylation of various downstream signaling molecules, thereby amplifying the T cell response. The importance of ZAP-70 in immune function is underscored by ZAP-70′s involvement in the development and activation of T cells, making ZAP-70 a vital component in the adaptive immune system. Dysregulation of ZAP-70 has been linked to immunodeficiencies and certain types of leukemia, highlighting ZAP-70′s significance in both health and disease. Anti-ZAP-70 antibody (3D2A4) is an invaluable tool for researchers studying T cell signaling pathways and their implications in immunological disorders.

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ZAP-70 Antibody (3D2A4) References:

1. Activation of Zap-70 tyrosine kinase due to a structural rearrangement induced by tyrosine phosphorylation and/or ITAM binding.  |  Visco, C., et al. 2000. Biochemistry. 39: 2784-91. PMID: 10704231
2. Flow cytometry for ZAP-70: New colors for chronic lymphocytic leukemia.  |  Wiestner, A. 2006. Cytometry B Clin Cytom. 70: 201-3. PMID: 16906579
3. ZAP-70 expression in B-cell chronic lymphocytic leukemia: evaluation by external (isotypic) or internal (T/NK cells) controls and correlation with IgV(H) mutations.  |  Zucchetto, A., et al. 2006. Cytometry B Clin Cytom. 70: 284-92. PMID: 16906587
4. ZAP-70 enhances B-cell-receptor signaling despite absent or inefficient tyrosine kinase activation in chronic lymphocytic leukemia and lymphoma B cells.  |  Gobessi, S., et al. 2007. Blood. 109: 2032-9. PMID: 17038529
5. Structural basis for activation of ZAP-70 by phosphorylation of the SH2-kinase linker.  |  Yan, Q., et al. 2013. Mol Cell Biol. 33: 2188-201. PMID: 23530057
6. Modification by covalent reaction or oxidation of cysteine residues in the tandem-SH2 domains of ZAP-70 and Syk can block phosphopeptide binding.  |  Visperas, PR., et al. 2015. Biochem J. 465: 149-61. PMID: 25287889
7. ZAP-70 Regulates Autoimmune Arthritis via Alterations in T Cell Activation and Apoptosis.  |  Kugyelka, R., et al. 2019. Cells. 8: PMID: 31137740
8. Clinical, Immunological, and Genetic Features in 49 Patients With ZAP-70 Deficiency: A Systematic Review.  |  Sharifinejad, N., et al. 2020. Front Immunol. 11: 831. PMID: 32431715
9. ZAP-70 constitutively regulates gene expression and protein synthesis in chronic lymphocytic leukemia.  |  Chen, J., et al. 2021. Blood. 137: 3629-3640. PMID: 33619528
10. An evolutionary divergent thermodynamic brake in ZAP-70 fine-tunes the kinetic proofreading in T cells.  |  Gangopadhyay, K., et al. 2022. J Biol Chem. 298: 102376. PMID: 35970395

The preferred ZAP-70 antibody is ZAP-70 Antibody (1E7.2): sc-32760.