YT521-B Antibody (8): sc-136428

YT521-B Antibody (8) is a mouse monoclonal IgG1 kappa light chain antibody that detects YT521-B protein of human, rat, mouse, and canine origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). YT521-B (8) antibody is available in both non-conjugated and various conjugated forms, including agarose and horseradish peroxidase (HRP). YT521-B protein, also known as YTH domain-containing protein 1, is a 727 amino acid nuclear protein that plays a crucial role in gene expression regulation and alternative splicing. YT521-B protein is localized to unique subnuclear structures known as YT bodies and is the human homolog of the mouse gene Ythdc1. YT521-B protein is ubiquitously expressed across tissues and is involved in a signal transduction pathway that influences splice site selection, which is vital for proper gene expression and protein diversity generation. YT521-B protein shuttles between the nucleus and cytosol, where phosphorylation occurs by kinases such as c-Src and Fyn. Tyrosine phosphorylation by c-Abl leads to YT521-B protein dispersion from YT bodies into the nucleoplasm and promotes sequestration in an insoluble nuclear form, inhibiting alternative splice site modulation. This regulatory mechanism is particularly important for Emery-Dreifuss muscular dystrophy (EDMD), where YT521-B protein is considered a candidate for involvement in the pathogenesis of this condition affecting voluntary muscles. YT521-B protein exists as two isoforms due to alternative splicing events, further highlighting its complexity and significance in cellular processes.

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YT521-B Antibody (8) References:

1. The interaction and colocalization of Sam68 with the splicing-associated factor YT521-B in nuclear dots is regulated by the Src family kinase p59(fyn).  |  Hartmann, AM., et al. 1999. Mol Biol Cell. 10: 3909-26. PMID: 10564280
2. The ER repeat protein YT521-B localizes to a novel subnuclear compartment.  |  Nayler, O., et al. 2000. J Cell Biol. 150: 949-62. PMID: 10973987
3. The STAR/GSG family protein rSLM-2 regulates the selection of alternative splice sites.  |  Stoss, O., et al. 2001. J Biol Chem. 276: 8665-73. PMID: 11118435
4. YTH: a new domain in nuclear proteins.  |  Stoilov, P., et al. 2002. Trends Biochem Sci. 27: 495-7. PMID: 12368078
5. Emerin interacts in vitro with the splicing-associated factor, YT521-B.  |  Wilkinson, FL., et al. 2003. Eur J Biochem. 270: 2459-66. PMID: 12755701
6. The intranuclear localization and function of YT521-B is regulated by tyrosine phosphorylation.  |  Rafalska, I., et al. 2004. Hum Mol Genet. 13: 1535-49. PMID: 15175272
7. p59(fyn)-mediated phosphorylation regulates the activity of the tissue-specific splicing factor rSLM-1.  |  Stoss, O., et al. 2004. Mol Cell Neurosci. 27: 8-21. PMID: 15345239
8. Common variations in the IL4R gene affect splicing and influence natural expression of the soluble isoform.  |  Bergin, AM., et al. 2006. Hum Mutat. 27: 990-8. PMID: 16917945
9. Cloning of a gene, YT521, for a novel RNA splicing-related protein induced by hypoxia/reoxygenation.  |  Imai, Y., et al. 1998. Brain Res Mol Brain Res. 53: 33-40. PMID: 9473574

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-YT521-B Antibody (8) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-YT521-B Antibody (8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

YT521-B Antibody (8) Specifications

Alternate Product Name: YT521-B

Product Clone Name: 8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 6-123

Epitope Target: YT521-B

Epitope Species: rat

Product Reactivity/Detection: YT521-B of human and rat origin and YTHDC1 of mouse and canine origin

Species Reactivity/Detection: mouse, rat, human, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for YT521-B Antibody (8): AC: Agarose, HRP: Horseradish Peroxidase