YAP Antibody (B-8): sc-398182
- YAP Antibody (B-8) is a mouse monoclonal IgG2a κ, cited in 9 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 294-323 within an internal region of YAP of human origin
- Anti-YAP Antibody (B-8) is recommended for detection of YAP of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-YAP Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-398182 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for YAP Antibody (B-8) for WB applications. These reagents are now offered in bundles with YAP Antibody (B-8) (see ordering information below).
QUICK LINKS
SEE ALSO...
YAP Antibody (B-8) is a mouse monoclonal IgG2a kappa light chain antibody engineered to detect Yes-associated protein (YAP), also known as YAP1, across multiple species including mouse, rat, and human. YAP monoclonal antibody (B-8) targets a precise epitope mapping to amino acids 294-323 within an internal region of human YAP, ensuring reliable and accurate binding in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). YAP is a critical component of the Hippo signaling pathway, functioning as a transcriptional co-activator that regulates essential cellular processes including cell proliferation, apoptosis, and organ size, which are vital for maintaining tissue homeostasis and are implicated in various cancers when dysregulated. Post-translational modifications of YAP, such as phosphorylation at serine residues like Ser112, play a crucial role in controlling its interaction with 14-3-3 proteins, thereby regulating its localization between the cytoplasm and nucleus and modulating its activity in gene expression. These modifications are essential for linking extracellular signals to nuclear responses, influencing processes like cell differentiation and cycle progression. Additionally, YAP shares significant homology with TAZ (transcriptional co-activator with PDZ-binding motif), highlighting its importance in complex cellular signaling networks. The structural integrity and post-translational modifications of YAP are pivotal for its ability to interact with other proteins and transcription factors, thereby affecting various signaling pathways and cellular functions. Expressed abundantly in tissues such as the lung, placenta, prostate, ovary, and testis, anti-YAP antibody (B-8) is an indispensable tool for researchers aiming to explore the intricate mechanisms of YAP′s function and its role in both normal physiology and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
YAP Antibody (B-8) | sc-398182 | 200 µg/ml | $322.00 | |||
YAP Antibody (B-8): m-IgG Fc BP-HRP Bundle | sc-526293 | 200 µg Ab; 10 µg BP | $361.00 | |||
YAP Antibody (B-8): m-IgG2a BP-HRP Bundle | sc-546567 | 200 µg Ab; 10 µg BP | $361.00 | |||
YAP Antibody (B-8) X | sc-398182 X | 200 µg/0.1 ml | $322.00 | |||
YAP Antibody (B-8) AC | sc-398182 AC | 500 µg/ml, 25% agarose | $424.00 | |||
YAP Antibody (B-8) HRP | sc-398182 HRP | 200 µg/ml | $322.00 | |||
YAP Antibody (B-8) FITC | sc-398182 FITC | 200 µg/ml | $336.00 | |||
YAP Antibody (B-8) PE | sc-398182 PE | 200 µg/ml | $349.00 | |||
YAP Antibody (B-8) Alexa Fluor® 488 | sc-398182 AF488 | 200 µg/ml | $364.00 | |||
YAP Antibody (B-8) Alexa Fluor® 546 | sc-398182 AF546 | 200 µg/ml | $364.00 | |||
YAP Antibody (B-8) Alexa Fluor® 594 | sc-398182 AF594 | 200 µg/ml | $364.00 | |||
YAP Antibody (B-8) Alexa Fluor® 647 | sc-398182 AF647 | 200 µg/ml | $364.00 | |||
YAP Antibody (B-8) Alexa Fluor® 680 | sc-398182 AF680 | 200 µg/ml | $364.00 | |||
YAP Antibody (B-8) Alexa Fluor® 790 | sc-398182 AF790 | 200 µg/ml | $364.00 | |||
YAP (B-8) Neutralizing Peptide | sc-398182 P | 100 µg/0.5 ml | $69.00 |