XylT-I Antibody (E-5): sc-390671
- XylT-I Antibody (E-5) is a mouse monoclonal IgG1 κ XylT-I antibody provided at 200 µg/ml
- raised against amino acids 871-944 mapping near the C-terminus of XylT-I of human origin
- XylT-I Antibody (E-5) is recommended for detection of XylT-I of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-XylT-I Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for XylT-I Antibody (E-5) for WB applications. These reagents are now offered in bundles with XylT-I Antibody (E-5) (see ordering information below).
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XylT-I Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects XylT-I of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). XylT-I (E-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Xylosyltransferase-I (XylT-I), also known as UDP-D-xylose:proteoglycan core protein β-D-xylosyltransferase 1, plays a crucial role in glycosaminoglycan chain biosynthesis, which are essential components of proteoglycans. XylT-I catalyzes UDP-xylose transfer to serine residues within specific recognition sequences on target proteins, a process vital for proper formation and function of extracellular matrix components. XylT-I shows varying expression levels across tissues, with higher levels in placenta, kidney, and pancreas, and lower levels in skeletal muscle. XylT-I is also involved in chondrocyte and tumor cell activity, highlighting its significance in both normal physiology and pathological conditions. XylT-I presence in diverse biological contexts underscores its potential as a target for therapeutic interventions and research applications.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
XylT-I Antibody (E-5) References:
- Analysis of the DXD motifs in human xylosyltransferase I required for enzyme activity. | Götting, C., et al. 2004. J Biol Chem. 279: 42566-73. PMID: 15294915
- Human xylosyltransferase I: functional and biochemical characterization of cysteine residues required for enzymic activity. | Müller, S., et al. 2005. Biochem J. 386: 227-36. PMID: 15461586
- Serum xylosyltransferase I activity, the new biochemical fibrosis marker, is not affected by renal insufficiency. | Schöttler, M., et al. 2005. Clin Biochem. 38: 486-8. PMID: 15820781
- Xylosyltransferase I acceptor properties of fibroblast growth factor and its fragment bFGF (1-24). | Kuhn, J., et al. 2005. Biochem Biophys Res Commun. 333: 156-66. PMID: 15936726
- Elevated xylosyltransferase I activities in pseudoxanthoma elasticum (PXE) patients as a marker of stimulated proteoglycan biosynthesis. | Götting, C., et al. 2005. J Mol Med (Berl). 83: 984-92. PMID: 16133423
- Impact of polymorphisms in the genes encoding xylosyltransferase I and a homologue in type 1 diabetic patients with and without nephropathy. | Schön, S., et al. 2005. Kidney Int. 68: 1483-90. PMID: 16164625
- Human xylosyltransferase I and N-terminal truncated forms: functional characterization of the core enzyme. | Müller, S., et al. 2006. Biochem J. 394: 163-71. PMID: 16225459
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XylT-I Antibody (E-5) | sc-390671 | 200 µg/ml | $322.00 | |||
XylT-I Antibody (E-5): m-IgG Fc BP-HRP Bundle | sc-530221 | 200 µg Ab; 10 µg BP | $361.00 | |||
XylT-I Antibody (E-5): m-IgGκ BP-HRP Bundle | sc-523630 | 200 µg Ab, 40 µg BP | $361.00 | |||
XylT-I Antibody (E-5): m-IgG1 BP-HRP Bundle | sc-543938 | 200 µg Ab; 20 µg BP | $361.00 | |||
XylT-I Antibody (E-5) AC | sc-390671 AC | 500 µg/ml, 25% agarose | $424.00 | |||
XylT-I Antibody (E-5) HRP | sc-390671 HRP | 200 µg/ml | $322.00 | |||
XylT-I Antibody (E-5) FITC | sc-390671 FITC | 200 µg/ml | $336.00 | |||
XylT-I Antibody (E-5) PE | sc-390671 PE | 200 µg/ml | $349.00 | |||
XylT-I Antibody (E-5) Alexa Fluor® 488 | sc-390671 AF488 | 200 µg/ml | $364.00 | |||
XylT-I Antibody (E-5) Alexa Fluor® 546 | sc-390671 AF546 | 200 µg/ml | $364.00 | |||
XylT-I Antibody (E-5) Alexa Fluor® 594 | sc-390671 AF594 | 200 µg/ml | $364.00 | |||
XylT-I Antibody (E-5) Alexa Fluor® 647 | sc-390671 AF647 | 200 µg/ml | $364.00 | |||
XylT-I Antibody (E-5) Alexa Fluor® 680 | sc-390671 AF680 | 200 µg/ml | $364.00 | |||
XylT-I Antibody (E-5) Alexa Fluor® 790 | sc-390671 AF790 | 200 µg/ml | $364.00 |