XPA Antibody (5A2): sc-53468
- XPA Antibody (5A2) is a mouse monoclonal mouse IgG1 κ κ provided at 200 µg/ml
- raised against His-tagged recombinant human XPA protein
- recommended for detection of XPA of human origin by WB, IP and IF
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for XPA Antibody (5A2). This work is in progress.
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XPA Antibody (5A2) is a mouse monoclonal IgG1 antibody that detects Xeroderma pigmentosum group A (XPA) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). XPA plays a critical role in the nucleotide excision repair (NER) pathway, which is essential for repairing DNA damage caused by ultraviolet (UV) light. XPA is primarily located in the nucleus, where XPA coordinates the assembly of a preincision complex necessary for the effective processing of damaged DNA. XPA′s nuclear localization ensures that repair machinery is positioned precisely where DNA damage occurs, thereby preventing the accumulation of mutations that can lead to skin cancers, particularly in individuals with xeroderma pigmentosum. XPA′s ability to form a homodimer in the absence of DNA and enhanced binding efficiency when dimerically bound underscores the regulatory mechanisms that cells employ to modulate activity based on DNA damage levels. Deficiencies in XPA result in a compromised ability to repair UV-induced DNA lesions, significantly increasing the risk of skin cancer in affected individuals.
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XPA Antibody (5A2) References:
- Coordinated regulation of XPA stability by ATR and HERC2 during nucleotide excision repair. | Lee, TH., et al. 2014. Oncogene. 33: 19-25. PMID: 23178497
- Redefining the DNA-binding domain of human XPA. | Sugitani, N., et al. 2014. J Am Chem Soc. 136: 10830-3. PMID: 25056193
- The RASSF1A tumor suppressor regulates XPA-mediated DNA repair. | Donninger, H., et al. 2015. Mol Cell Biol. 35: 277-87. PMID: 25368379
- Downregulated XPA promotes carcinogenesis of bladder cancer via impairment of DNA repair. | Zhi, Y., et al. 2017. Tumour Biol. 39: 1010428317691679. PMID: 28222669
- Association of XPA polymorphism with breast cancer risk: A meta-analysis. | Zhang, Y., et al. 2018. Medicine (Baltimore). 97: e11276. PMID: 29953005
- XPA serves as an autophagy and apoptosis inducer by suppressing hepatocellular carcinoma in a PI3K/Akt/mTOR dependent manner. | Deng, Y., et al. 2021. J Gastrointest Oncol. 12: 1797-1810. PMID: 34532129
- Idarubicin combats abiraterone and enzalutamide resistance in prostate cells via targeting XPA protein. | Zhang, Y., et al. 2022. Cell Death Dis. 13: 1034. PMID: 36509750
- Small Molecule Antagonists of the DNA Repair ERCC1/XPA Protein-Protein Interaction. | Obermann, R., et al. 2024. ChemMedChem. 19: e202300648. PMID: 38300970
- Generation of site-specifically labelled fluorescent human XPA to investigate DNA binding dynamics during nucleotide excision repair. | Kuppa, S., et al. 2024. Methods. 224: 47-53. PMID: 38387709
- XPA tumor variant leads to defects in NER that sensitize cells to cisplatin. | Blee, AM., et al. 2024. NAR Cancer. 6: zcae013. PMID: 38500596
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XPA Antibody (5A2) | sc-53468 | 200 µg/ml | $322.00 |