XPA Antibody (3F396): sc-73273
- XPA Antibody (3F396) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against full length XPA of human origin
- recommended for detection of XPA of mouse, rat and human origin by WB, IP, IF and IHC(P)
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for XPA Antibody (3F396) for WB and IHC(P) applications. This reagent is now offered in a bundle with XPA Antibody (3F396) (see ordering information below).
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XPA Antibody (3F396) is a mouse monoclonal IgG2a antibody that detects Xeroderma pigmentosum group A (XPA) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. XPA plays a crucial role in the nucleotide excision repair (NER) pathway, which is essential for repairing DNA damage caused by ultraviolet (UV) light. XPA is primarily located in the nucleus, where XPA coordinates the assembly of a preincision complex necessary for the recognition and excision of damaged DNA. XPA′s nuclear localization is directly involved in maintaining genomic stability; without proper localization and function, cells become increasingly susceptible to mutations and the development of skin cancers, particularly in individuals with xeroderma pigmentosum. XPA′s ability to form a homodimer in the absence of DNA and enhanced binding efficiency when dimerically bound underscores the regulatory mechanisms that cells employ to modulate activity based on DNA damage levels. Anti-XPA antibody (3F396) serves as an invaluable tool for researchers studying DNA repair mechanisms and the implications of XPA deficiencies in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
XPA Antibody (3F396) References:
- Human XPA and RPA DNA repair proteins participate in specific recognition of triplex-induced helical distortions. | Vasquez, KM., et al. 2002. Proc Natl Acad Sci U S A. 99: 5848-53. PMID: 11972036
- Additive roles of XPA and MSH2 genes in UVB-induced skin tumorigenesis in mice. | Yoshino, M., et al. 2002. DNA Repair (Amst). 1: 935-40. PMID: 12531021
- XPA gene-deficient, SCF-transgenic mice with epidermal melanin are resistant to UV-induced carcinogenesis. | Yamazaki, F., et al. 2004. J Invest Dermatol. 123: 220-8. PMID: 15191564
- XPA impacts formation but not proteasome-sensitive repair of DNA-protein cross-links induced by chromate. | Zecevic, A., et al. 2010. Mutagenesis. 25: 381-8. PMID: 20410141
- SIRT1 regulates UV-induced DNA repair through deacetylating XPA. | Fan, W. and Luo, J. 2010. Mol Cell. 39: 247-58. PMID: 20670893
- Poly(ADP-ribose)-mediated interplay of XPA and PARP1 leads to reciprocal regulation of protein function. | Fischer, JM., et al. 2014. FEBS J. 281: 3625-41. PMID: 24953096
- TP53 mutations induced by BPDE in Xpa-WT and Xpa-Null human TP53 knock-in (Hupki) mouse embryo fibroblasts. | Kucab, JE., et al. 2015. Mutat Res. 773: 48-62. PMID: 25847421
- HMGB1 interacts with XPA to facilitate the processing of DNA interstrand crosslinks in human cells. | Mukherjee, A. and Vasquez, KM. 2016. Nucleic Acids Res. 44: 1151-60. PMID: 26578599
- CXCL1 Inhibition Regulates UVB-Induced Skin Inflammation and Tumorigenesis in Xpa-Deficient Mice. | Kunisada, M., et al. 2017. J Invest Dermatol. 137: 1975-1983. PMID: 28528167
- The Distinct Roles of NEIL1 and XPA in Limiting Aflatoxin B1-Induced Mutagenesis in Mice. | Luzadder, MM., et al. 2025. Mol Cancer Res. 23: 46-58. PMID: 39387543
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XPA Antibody (3F396) | sc-73273 | 200 µg/ml | $316.00 | |||
XPA Antibody (3F396): m-IgG Fc BP-HRP Bundle | sc-551970 | 200 µg Ab; 10 µg BP | $354.00 |