Xinα Antibody (D-8): sc-166658
- Xinα Antibody (D-8) is a mouse monoclonal IgG1 κ Xinα antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 735-1034 mapping within an internal region of Xinα of human origin
- Xin alpha Antibody (D-8) is recommended for detection of Xinα of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Xin alpha Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Xinα Antibody (D-8) for WB applications. These reagents are now offered in bundles with Xinα Antibody (D-8) (see ordering information below).
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Xinα Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Xinα protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Xinα (D-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Xinα protein, also known as CMYA1 (Cardiomyopathy-associated protein 1) or XIRP1 (xin actin-binding repeat containing 1), is a crucial 1,843 amino acid protein that plays a vital role in protecting actin filaments from depolymerization, maintaining cytoskeleton integrity. Xinα protein is primarily located at cell junctions alongside actin stress fibers, containing 15 Xin repeats essential for stabilizing actin-based structures and facilitating microfilament crosslinking with actin networks. Xinα protein interacts with several key proteins, including β-catenin, Filamin, and VASP (vasodilator-stimulated phosphoprotein), important for various cellular processes. Xinα protein shares 78% similarity with mouse counterpart and is expressed in heart as three alternatively spliced isoforms designated A, B, and C. In mice, Xinα protein is essential for proper heart tube formation and correct cardiac looping, indicating human homolog may have similar critical functions in cardiac development and health.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Xinα Antibody (D-8) References:
- Requirement of a novel gene, Xin, in cardiac morphogenesis. | Wang, DZ., et al. 1999. Development. 126: 1281-94. PMID: 10021346
- Localization of the novel Xin protein to the adherens junction complex in cardiac and skeletal muscle during development. | Sinn, HW., et al. 2002. Dev Dyn. 225: 1-13. PMID: 12203715
- Xin repeats define a novel actin-binding motif. | Pacholsky, D., et al. 2004. J Cell Sci. 117: 5257-68. PMID: 15454575
- Unusual splicing events result in distinct Xin isoforms that associate differentially with filamin c and Mena/VASP. | van der Ven, PF., et al. 2006. Exp Cell Res. 312: 2154-67. PMID: 16631741
- Structure, Expression, and Function of a Novel Intercalated Disc Protein, Xin. | Jung-Ching Lin, J., et al. 2005. J Med Sci. 25: 215-222. PMID: 16708114
- The consensus coding sequences of human breast and colorectal cancers. | Sjöblom, T., et al. 2006. Science. 314: 268-74. PMID: 16959974
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Xinα Antibody (D-8) | sc-166658 | 200 µg/ml | $322.00 | |||
Xinα Antibody (D-8): m-IgG Fc BP-HRP Bundle | sc-529021 | 200 µg Ab; 10 µg BP | $361.00 | |||
Xinα Antibody (D-8): m-IgGκ BP-HRP Bundle | sc-521696 | 200 µg Ab, 40 µg BP | $361.00 | |||
Xinα Antibody (D-8): m-IgG1 BP-HRP Bundle | sc-543273 | 200 µg Ab; 20 µg BP | $361.00 | |||
Xinα Antibody (D-8) AC | sc-166658 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Xinα Antibody (D-8) HRP | sc-166658 HRP | 200 µg/ml | $322.00 | |||
Xinα Antibody (D-8) FITC | sc-166658 FITC | 200 µg/ml | $336.00 | |||
Xinα Antibody (D-8) PE | sc-166658 PE | 200 µg/ml | $349.00 | |||
Xinα Antibody (D-8) Alexa Fluor® 488 | sc-166658 AF488 | 200 µg/ml | $364.00 | |||
Xinα Antibody (D-8) Alexa Fluor® 546 | sc-166658 AF546 | 200 µg/ml | $364.00 | |||
Xinα Antibody (D-8) Alexa Fluor® 594 | sc-166658 AF594 | 200 µg/ml | $364.00 | |||
Xinα Antibody (D-8) Alexa Fluor® 647 | sc-166658 AF647 | 200 µg/ml | $364.00 | |||
Xinα Antibody (D-8) Alexa Fluor® 680 | sc-166658 AF680 | 200 µg/ml | $364.00 | |||
Xinα Antibody (D-8) Alexa Fluor® 790 | sc-166658 AF790 | 200 µg/ml | $364.00 |