XAF1 Antibody (H-4): sc-374378

XAF1 Antibody (H-4) is a mouse monoclonal IgG1 antibody that detects XAF1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). XAF1, or X-linked inhibitor of apoptosis protein (XIAP)-associated factor 1, plays a crucial role in regulating apoptosis by inhibiting the anti-apoptotic activity of XIAP, a member of the inhibitor of apoptosis protein (IAP) family that prevents programmed cell death by inhibiting caspases. The importance of XAF1 lies in its ability to promote apoptosis in cells where XIAP is overexpressed, which is often the case in various cancers. By redistributing XIAP from the cytosol to the nucleus in the presence of XAF1, the balance between cell survival and death is shifted, making XAF1 a potential target for therapeutic intervention in cancer treatment. Notably, XAF1 transcripts are highly expressed in heart and ovary tissues, and low levels of XAF1 mRNA have been associated with increased cancer cell survival, indicating that enhancing XAF1 expression could restore apoptotic pathways in tumor cells. Furthermore, the human XAF1 gene is activated by interferons, specifically IFN-α and IFN-β, highlighting XAF1′s role in immune response and potential implications in cancer immunotherapy. Anti-XAF1 antibody (H-4) is an essential tool for researchers studying apoptosis and its regulation in human health and disease.

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XAF1 Antibody (H-4) References:

1. Xaf1 can cooperate with TNFalpha in the induction of apoptosis, independently of interaction with XIAP.  |  Xia, Y., et al. 2006. Mol Cell Biochem. 286: 67-76. PMID: 16432762
2. Silencing of the XAF1 gene by promoter hypermethylation in cancer cells and reactivation to TRAIL-sensitization by IFN-beta.  |  Micali, OC., et al. 2007. BMC Cancer. 7: 52. PMID: 17376236
3. Tumor suppressor XAF1 induces apoptosis, inhibits angiogenesis and inhibits tumor growth in hepatocellular carcinoma.  |  Zhu, LM., et al. 2014. Oncotarget. 5: 5403-15. PMID: 24980821
4. XAF1 promotes neuroblastoma tumor suppression and is required for KIF1Bβ-mediated apoptosis.  |  Choo, Z., et al. 2016. Oncotarget. 7: 34229-39. PMID: 27097110
5. XAF1 forms a positive feedback loop with IRF-1 to drive apoptotic stress response and suppress tumorigenesis.  |  Jeong, SI., et al. 2018. Cell Death Dis. 9: 806. PMID: 30042418
6. XAF1 destabilizes estrogen receptor α through the assembly of a BRCA1-mediated destruction complex and promotes estrogen-induced apoptosis.  |  Lim, JS., et al. 2022. Oncogene. 41: 2897-2908. PMID: 35430604
7. XAF1 drives apoptotic switch of endoplasmic reticulum stress response through destabilization of GRP78 and CHIP.  |  Lee, KW., et al. 2022. Cell Death Dis. 13: 655. PMID: 35902580
8. XAF1 Protects Host against Emerging RNA Viruses by Stabilizing IRF1-Dependent Antiviral Immunity.  |  Han, Y., et al. 2022. J Virol. 96: e0077422. PMID: 35972291
9. XAF1 promotes colorectal cancer metastasis via VCP-RNF114-JUP axis.  |  Xia, J., et al. 2024. J Cell Biol. 223: PMID: 38095639
10. XAF1 antagonizes TRIM28 activity through the assembly of a ZNF313-mediated destruction complex to suppress tumor malignancy.  |  Jang, SH., et al. 2024. Mol Biomed. 5: 58. PMID: 39532800

The preferred XAF1 antibody is XAF1 Antibody (B-5): sc-398012.