XAF1 Antibody (B-5): sc-398012

XAF1 Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects XAF1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). XAF1 Antibody (B-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The X-linked inhibitor of apoptosis protein (XIAP)-associated factor 1 (XAF1) plays a crucial role in regulating apoptosis by inhibiting the anti-apoptotic activity of XIAP, a member of the inhibitor of apoptosis protein (IAP) family that prevents programmed cell death by inhibiting caspases. XAF1 promotes apoptosis in cells, particularly in cancer, where low XAF1 expression can lead to enhanced survival of malignant cells due to increased XIAP activity. XAF1 is predominantly expressed in the heart and ovary, with transcripts of varying lengths (3.9-, 4.5-, 6.0-, and 7.0-kb) detected at high levels in these tissues. Additionally, interferon-alpha and interferon-beta activate the human XAF1 gene, highlighting XAF1′s role in immune responses, as XAF1 maps to chromosome 17p13.2, suggesting a potential link between XAF1 expression and apoptosis regulation in immune cells.

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XAF1 Antibody (B-5) References:

1. Expression and genetic analysis of XIAP-associated factor 1 (XAF1) in cancer cell lines.  |  Fong, WG., et al. 2000. Genomics. 70: 113-22. PMID: 11087668
2. Identification of XAF1 as an antagonist of XIAP anti-Caspase activity.  |  Liston, P., et al. 2001. Nat Cell Biol. 3: 128-33. PMID: 11175744
3. XIAP: apoptotic brake and promising therapeutic target.  |  Holcik, M., et al. 2001. Apoptosis. 6: 253-61. PMID: 11445667
4. IAPs are essential for GDNF-mediated neuroprotective effects in injured motor neurons in vivo.  |  Perrelet, D., et al. 2002. Nat Cell Biol. 4: 175-9. PMID: 11813002
5. Identification of X-linked inhibitor of apoptosis-associated factor-1 as an interferon-stimulated gene that augments TRAIL Apo2L-induced apoptosis.  |  Leaman, DW., et al. 2002. J Biol Chem. 277: 28504-11. PMID: 12029096
6. XAF1 mediates apoptosis through an extracellular signal-regulated kinase pathway in colon cancer.  |  Yu, LF., et al. 2007. Cancer. 109: 1996-2003. PMID: 17385215
7. XAF1 is frequently methylated in human esophageal cancer.  |  Chen, XY., et al. 2012. World J Gastroenterol. 18: 2844-9. PMID: 22719195
8. Experimental study on inhibition effects of the XAF1 gene against lung cancer cell proliferation.  |  Yang, WT., et al. 2014. Asian Pac J Cancer Prev. 15: 7825-9. PMID: 25292071
9. XAF1 directs apoptotic switch of p53 signaling through activation of HIPK2 and ZNF313.  |  Lee, MG., et al. 2014. Proc Natl Acad Sci U S A. 111: 15532-7. PMID: 25313037
10. Identification of XAF1-MT2A mutual antagonism as a molecular switch in cell-fate decisions under stressful conditions.  |  Shin, CH., et al. 2017. Proc Natl Acad Sci U S A. 114: 5683-5688. PMID: 28507149
11. XAF1 overexpression inhibits the malignant progression and cisplatin resistance of NSCLC by activating endoplasmic reticulum stress.  |  Chen, B., et al. 2024. Mol Biol Rep. 51: 435. PMID: 38520543
12. XAF1 is secreted from stressed tumor cells to activate T cell-mediated tumor surveillance via Lck-ERK signaling.  |  Ahn, J., et al. 2025. Neoplasia. 59: 101094. PMID: 39615106

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-XAF1 Antibody (B-5) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-XAF1 Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

XAF1 Antibody (B-5) Specifications

Alternate Product Name: XAF1

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-225

Epitope Target: XAF1

Epitope Species: human

Product Reactivity/Detection: XAF1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for XAF1 Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin