WTAP Antibody (D-7): sc-374280

WTAP Antibody (D-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects WTAP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). WTAP Antibody (D-7) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Wilms′ tumor 1-associating protein (WTAP) plays a crucial role in mRNA splicing regulation and is predominantly located in nuclear speckles, which are essential for pre-mRNA processing and storage. WTAP′s nuclear speckle localization enables interactions with splicing factors and regulatory proteins, influencing gene expression and cellular responses during G2/M cell cycle transition. WTAP enhances cyclin A2 stability by binding to its 3′ untranslated region, which is vital for proper cell cycle progression. WTAP inhibits WT1 target gene expression and can impair WT1′s DNA-binding ability, highlighting WTAP′s regulatory importance in cellular processes. Alternative splicing events produce two WTAP isoforms, adding complexity to WTAP′s function in cellular regulation.

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WTAP Antibody (D-7) References:

1. A novel protein interacts with the Werner's syndrome gene product physically and functionally.  |  Kawabe Yi,., et al. 2001. J Biol Chem. 276: 20364-9. PMID: 11301316
2. Exclusion of WTAP and HOXA13 as candidate genes for isolated hypospadias.  |  Utsch, B., et al. 2003. Scand J Urol Nephrol. 37: 498-501. PMID: 14675924
3. Immunohistochemical expression of Wilms' tumor 1 protein in nephroblastoma.  |  Chen, BF., et al. 2004. J Chin Med Assoc. 67: 506-10. PMID: 15648285
4. Wilms' tumor 1 and signal transducers and activators of transcription 3 synergistically promote cell proliferation: a possible mechanism in sporadic Wilms' tumor.  |  Rong, Y., et al. 2006. Cancer Res. 66: 8049-57. PMID: 16912181
5. Wilms' tumor 1-associating protein regulates G2/M transition through stabilization of cyclin A2 mRNA.  |  Horiuchi, K., et al. 2006. Proc Natl Acad Sci U S A. 103: 17278-83. PMID: 17088532
6. Wilms' tumor 1-associating protein regulates the proliferation of vascular smooth muscle cells.  |  Small, TW., et al. 2006. Circ Res. 99: 1338-46. PMID: 17095724
7. Wilms' tumor protein Wt1 is an activator of the anti-Müllerian hormone receptor gene Amhr2.  |  Klattig, J., et al. 2007. Mol Cell Biol. 27: 4355-64. PMID: 17420277
8. Wilms' tumor protein and FLT3-internal tandem duplication expression in patients with de novo acute myeloid leukemia.  |  Spassov, BV., et al. 2011. Hematology. 16: 37-42. PMID: 21269566
9. Wilms' tumor protein 1 (WT1) peptide vaccination in AML patients: predominant TCR CDR3β sequence associated with remission in one patient is detectable in other vaccinated patients.  |  Ochsenreither, S., et al. 2012. Cancer Immunol Immunother. 61: 313-22. PMID: 21898091
10. Immunohistochemical expression of Wilms' tumor protein (WT1) in developing human epithelial and mesenchymal tissues.  |  Parenti, R., et al. 2013. Acta Histochem. 115: 70-5. PMID: 22673530
11. The Wilms tumor protein WT1 stimulates transcription of the gene encoding insulin-like growth factor binding protein 5 (IGFBP5).  |  Müller, M., et al. 2017. Gene. 619: 21-29. PMID: 28366834
12. Wilms tumor protein-dependent transcription of VEGF receptor 2 and hypoxia regulate expression of the testis-promoting gene Sox9 in murine embryonic gonads.  |  Kirschner, KM., et al. 2017. J Biol Chem. 292: 20281-20291. PMID: 29042436

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-WTAP Antibody (D-7) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-WTAP Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

WTAP Antibody (D-7) Specifications

Alternate Product Name: WTAP

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 371-399

Epitope Target: WTAP

Epitope Species: human

Product Reactivity/Detection: WTAP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for WTAP Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin