Wnt-7a CRISPR/Cas9 KO Plasmid (m): sc-423721
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Wnt-7a CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Wnt-7a genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Wnt-7a Antibody (E-9): sc-365665
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Wnt-7a CRISPR/Cas9 KO Plasmid (m) | sc-423721 | 20 µg | $397.00 |
Overview
Wnt7a encodes the secreted ligand Wnt-7a, a key regulator of Wnt signaling that influences β-catenin–dependent transcription as well as non-canonical pathway outputs controlling cytoskeletal dynamics and planar cell polarity. In mouse tissues, Wnt-7a contributes to embryonic patterning and organogenesis and helps coordinate cell fate specification, proliferation, and migration through Frizzled/LRP receptor engagement. Altered Wnt7a activity is frequently studied in contexts of aberrant developmental programs and dysregulated tissue homeostasis where Wnt pathway tone and feedback loops shape gene expression states. As a pathway node with broad downstream effects, Wnt-7a is commonly investigated for its roles in morphogenesis, stem/progenitor cell behavior, and remodeling of tissue architecture.
Wnt-7a CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Wnt7a gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Wnt7a together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Wnt7a open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Wnt-7a protein expression.
This CRISPR knockout system enables efficient generation of Wnt7a-deficient cell models for investigation of Wnt-7a signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Wnt7a exon(s) critical for Wnt-7a function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Wnt7a genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Wnt-7a CRISPR/Cas9 KO Plasmid (m) and Wnt-7a CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Wnt7a locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Wnt-7a HDR Plasmid (m) and Wnt-7a HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Wnt7a homology arms to support homology-directed repair at defined Wnt7a target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.