Wnt-7a/b Antibody (H-8): sc-365459

Wnt-7a/b Antibody (H-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Wnt-7a/b protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Wnt-7a/b antibody (H-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Wnt-7a/b plays a crucial role in regulating developmental processes, particularly in forming and maintaining the female reproductive tract. Wnt-7a is essential for proper anterior-posterior axis development, influencing uterine smooth muscle patterning and ensuring adult uterus functionality. Wnt-7a/b signaling is vital for cell fate determination during embryogenesis, and dysregulation can lead to developmental abnormalities and contribute to oncogenesis. Human Wnt-7a gene is located on chromosome 3p25, while Wnt-7b gene is found on chromosome 22q13, highlighting their genetic significance in both normal physiology and disease states.

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Wnt-7a/b Antibody (H-8) References:

1. The Wnt/Ca2+ pathway: a new vertebrate Wnt signaling pathway takes shape.  |  Kühl, M., et al. 2000. Trends Genet. 16: 279-83. PMID: 10858654
2. Decreased expression of Wnt7a mRNA is inversely associated with the expression of estrogen receptor-alpha in human uterine leiomyoma.  |  Li, S., et al. 2001. J Clin Endocrinol Metab. 86: 454-7. PMID: 11232041
3. Wnt-7a causes loss of differentiated phenotype and inhibits apoptosis of articular chondrocytes via different mechanisms.  |  Hwang, SG., et al. 2004. J Biol Chem. 279: 26597-604. PMID: 15082716
4. Restoration of Wnt-7a expression reverses non-small cell lung cancer cellular transformation through frizzled-9-mediated growth inhibition and promotion of cell differentiation.  |  Winn, RA., et al. 2005. J Biol Chem. 280: 19625-34. PMID: 15705594
5. BMP-2-enhanced chondrogenesis involves p38 MAPK-mediated down-regulation of Wnt-7a pathway.  |  Jin, EJ., et al. 2006. Mol Cells. 22: 353-9. PMID: 17202865
6. Expression of MIG-6, WNT-9A, and WNT-7B during osteoarthritis.  |  Velasquillo, C., et al. 2007. Ann N Y Acad Sci. 1117: 175-80. PMID: 18056042
7. Wnt-7a Stimulates Dendritic Spine Morphogenesis and PSD-95 Expression Through Canonical Signaling.  |  Ramos-Fernández, E., et al. 2019. Mol Neurobiol. 56: 1870-1882. PMID: 29967987
8. Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410.  |  Sun, WL., et al. 2019. Biosci Rep. 39: PMID: 30498093
9. Isolation, characterization and chromosomal assignment of the human WNT7A gene.  |  Ikegawa, S., et al. 1996. Cytogenet Cell Genet. 74: 149-52. PMID: 8893824
10. Molecular models for vertebrate limb development.  |  Johnson, RL. and Tabin, CJ. 1997. Cell. 90: 979-90. PMID: 9323126
11. WNT-7a induces axonal remodeling and increases synapsin I levels in cerebellar neurons.  |  Lucas, FR. and Salinas, PC. 1997. Dev Biol. 192: 31-44. PMID: 9405095
12. Inhibition of GSK-3beta leading to the loss of phosphorylated MAP-1B is an early event in axonal remodelling induced by WNT-7a or lithium.  |  Lucas, FR., et al. 1998. J Cell Sci. 111 (Pt 10): 1351-61. PMID: 9570753

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Wnt-7a/b Antibody (H-8) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Wnt-7a/b Antibody (H-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Wnt-7a/b Antibody (H-8) Specifications

Alternate Product Name: WNT7A/B

Product Clone Name: H-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 32-71

Epitope Target: Wnt-7a

Epitope Species: human

Product Reactivity/Detection: precursor and mature Wnt-7a and Wnt-7b

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Wnt-7a/b Antibody (H-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Wnt-7a antibody is Wnt-7a Antibody (E-9): sc-365665.