Wnt-2b CRISPR/Cas9 KO Plasmid (m): sc-423714
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Wnt-2b CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Wnt-2b genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Wnt-2b Antibody (C-2): sc-166502
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Wnt-2b CRISPR/Cas9 KO Plasmid (m) | sc-423714 | 20 µg | $397.00 |
Overview
Wnt2b encodes the secreted glycoprotein Wnt-2b, a ligand that activates Frizzled/LRP receptor complexes to regulate canonical β-catenin signaling and, in some contexts, non-canonical Wnt pathways. In mouse tissues, Wnt-2b contributes to cell fate specification, proliferation control, epithelial–mesenchymal interactions, and organ morphogenesis, with prominent roles reported in ocular and forebrain development. By modulating transcriptional programs downstream of TCF/LEF, Wnt-2b influences stem/progenitor cell maintenance and tissue patterning. Dysregulated Wnt2b signaling is relevant to studies of developmental defects and Wnt-driven pathobiology, including mechanisms linked to aberrant growth and differentiation.
Wnt-2b CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Wnt2b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Wnt2b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Wnt2b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Wnt-2b protein expression.
This CRISPR knockout system enables efficient generation of Wnt2b-deficient cell models for investigation of Wnt-2b signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Wnt2b exon(s) critical for Wnt-2b function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Wnt2b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Wnt-2b CRISPR/Cas9 KO Plasmid (m) and Wnt-2b CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Wnt2b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Wnt-2b HDR Plasmid (m) and Wnt-2b HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Wnt2b homology arms to support homology-directed repair at defined Wnt2b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.