Wnt-16 Antibody (B-4): sc-271897

Wnt-16 Antibody (B-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Wnt-16 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Wnt-16 antibody (B-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Wnt-16 is a member of the Wnt family of secreted glycoproteins, which are crucial for numerous developmental processes, including the regulation of cell growth and differentiation. Notably, Wnt-16 has two mRNA isoforms, Wnt-16a and Wnt-16b, which exhibit distinct expression patterns; Wnt-16a is primarily found in the pancreas, while Wnt-16b is abundantly expressed in adult kidney, placenta, brain, heart, and spleen, but not in bone marrow. The differential expression of these isoforms suggests a specialized role for Wnt-16b in organ-specific functions and potential implications in developmental biology and disease. Wnt-16 is implicated in the pathogenesis of certain leukemias, serving as a downstream target of the E2A-Pbx1 hybrid gene, indicating that Wnt-16-mediated autocrine signaling may play a role in the growth of pre-B acute lymphoblastoid leukemias. anti-Wnt-16 antibody (B-4) serves as a valuable tool for researchers investigating the Wnt signaling pathway and its involvement in cancer and developmental disorders.

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Wnt-16 Antibody (B-4) References:

1. Oncogenic homeodomain transcription factor E2A-Pbx1 activates a novel WNT gene in pre-B acute lymphoblastoid leukemia.  |  McWhirter, JR., et al. 1999. Proc Natl Acad Sci U S A. 96: 11464-9. PMID: 10500199
2. Wnt-16a, a novel Wnt-16 isoform, which shows differential expression in adult human tissues.  |  Fear, MW., et al. 2000. Biochem Biophys Res Commun. 278: 814-20. PMID: 11095990
3. Molecular cloning and characterization of WNT14B, a novel member of the WNT gene family.  |  Kirikoshi, H., et al. 2001. Int J Oncol. 19: 947-52. PMID: 11604992
4. Expression of WNT14 and WNT14B mRNAs in human cancer, up-regulation of WNT14 by IFNgamma and up-regulation of WNT14B by beta-estradiol.  |  Kirikoshi, H., et al. 2001. Int J Oncol. 19: 1221-5. PMID: 11713592
5. A new WNT on the bone: WNT16, cortical bone thickness, porosity and fractures.  |  Gori, F., et al. 2015. Bonekey Rep. 4: 669. PMID: 25987984
6. The bone-sparing effects of estrogen and WNT16 are independent of each other.  |  Movérare-Skrtic, S., et al. 2015. Proc Natl Acad Sci U S A. 112: 14972-7. PMID: 26627248
7. Wnt16 attenuates osteoarthritis progression through a PCP/JNK-mTORC1-PTHrP cascade.  |  Tong, W., et al. 2019. Ann Rheum Dis. 78: 551-561. PMID: 30745310
8. Wnt16 signaling in bone homeostasis and osteoarthristis.  |  Ye, X. and Liu, X. 2022. Front Endocrinol (Lausanne). 13: 1095711. PMID: 36619549
9. Multiple Mechanisms Explain Genetic Effects at the CPED1-WNT16 Bone Mineral Density Locus.  |  Gómez, AE., et al. 2023. Curr Osteoporos Rep. 21: 173-183. PMID: 36943599
10. Wnt16 Promotes Vascular Smooth Muscle Contractile Phenotype and Function via Taz (Wwtr1) Activation in Male LDLR-/- Mice.  |  Behrmann, A., et al. 2023. Endocrinology. 165: PMID: 38123514
11. Microgel Encapsulated Mesoporous Silica Nanoparticles for Releasing Wnt16 to Synergistically Treat Temporomandibular Joint Osteoarthritis.  |  Zhu, Y., et al. 2024. Adv Sci (Weinh). 11: e2404396. PMID: 39248388
12. Isolation of two novel WNT genes, WNT14 and WNT15, one of which (WNT15) is closely linked to WNT3 on human chromosome 17q21.  |  Bergstein, I., et al. 1997. Genomics. 46: 450-8. PMID: 9441749

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Wnt-16 Antibody (B-4) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-Wnt-16 Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Wnt-16 Antibody (B-4) Specifications

Alternate Product Name: WNT16

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 111-207

Epitope Target: Wnt-16

Epitope Species: human

Product Reactivity/Detection: Wnt-16

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for Wnt-16 Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin