WIP Antibody (A-7): sc-271113

WIP Antibody (A-7) is a mouse monoclonal IgG2b antibody that detects WIP in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). WIP, or WAS-interacting protein, plays a crucial role in the regulation of actin dynamics and is essential for proper immune cell function. WIP contains multiple domains that facilitate actin-binding and interaction with other signaling proteins, which are vital for T cell and B cell activation. WIP is predominantly located in the cytoplasm, where WIP associates with the Wiskott-Aldrich syndrome protein (WASP) to form a complex necessary for the activation of NF-AT/AP-1 transcription factors, critical for T cell responses. Disruption of this complex can lead to immunodeficiency, as seen in Wiskott-Aldrich syndrome, highlighting WIP′s importance in maintaining immune system integrity. The gene encoding human WIP is located on chromosome 2p23.3-q24.3, and WIP expression is tightly regulated, with overexpression linked to increased F-actin content and altered cellular morphology in B cells. Anti-WIP antibody (A-7) is an invaluable tool for researchers studying the molecular mechanisms underlying immune responses and the pathogenesis of immunodeficiencies.

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WIP Antibody (A-7) References:

1. WIP: WASP-interacting proteins at invadopodia and podosomes.  |  García, E., et al. 2012. Eur J Cell Biol. 91: 869-77. PMID: 22823953
2. WIP remodeling actin behind the scenes: how WIP reshapes immune and other functions.  |  Noy, E., et al. 2012. Int J Mol Sci. 13: 7629-7647. PMID: 22837718
3. WIP: more than a WASp-interacting protein.  |  Fried, S., et al. 2014. J Leukoc Biol. 96: 713-27. PMID: 25210148
4. WIP and WICH/WIRE co-ordinately control invadopodium formation and maturation in human breast cancer cell invasion.  |  García, E., et al. 2016. Sci Rep. 6: 23590. PMID: 27009365
5. Crosstalk between WIP and Rho family GTPases.  |  Antón, IM., et al. 2020. Small GTPases. 11: 160-166. PMID: 29172947
6. PD-L1 promotes tumor growth and progression by activating WIP and β-catenin signaling pathways and predicts poor prognosis in lung cancer.  |  Yu, W., et al. 2020. Cell Death Dis. 11: 506. PMID: 32632098
7. WIP Modulates Oxidative Stress through NRF2/KEAP1 in Glioblastoma Cells.  |  Escoll, M., et al. 2020. Antioxidants (Basel). 9: PMID: 32825452
8. Macrophage mitochondrial fission improves cancer cell phagocytosis induced by therapeutic antibodies and is impaired by glutamine competition.  |  Li, J., et al. 2022. Nat Cancer. 3: 453-470. PMID: 35484420
9. CLDN6 inhibits breast cancer metastasis through WIP-dependent actin cytoskeleton-mediated autophagy.  |  Dong, Y., et al. 2023. J Exp Clin Cancer Res. 42: 68. PMID: 36935496
10. Multifaceted role of the actin-binding protein WIP: Promotor and inhibitor of tumor progression and dissemination.  |  Alonso-Eiras, J. and Anton, IM. 2024. Cytoskeleton (Hoboken).. PMID: 39329352

The preferred WIP antibody is WIP Antibody (C-1): sc-390099.