WIF-1 Antibody (B-10): sc-373780

WIF-1 Antibody (B-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects WIF-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). WIF-1 (B-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. WIF-1 plays a crucial role as a Wnt antagonist, which is essential for regulating the Wnt signaling pathway that governs numerous developmental processes such as embryogenesis and central nervous system patterning. By inhibiting Wnt signaling, WIF-1 ensures that the spatial and temporal expression of Wnt target genes is properly controlled, preventing aberrant cell proliferation and differentiation that could lead to developmental disorders or cancer. Structurally, WIF-1 is a 379-amino acid secreted protein characterized by an N-terminal signal sequence, a 150-amino acid WIF domain, five epidermal growth factor-like repeats, and a 45-amino acid hydrophilic C-terminal domain, which collectively contribute to binding Wnt proteins and modulating their activity. Anti-WIF-1 antibody (B-10) versatility in various applications makes this product an invaluable tool for researchers studying the intricate mechanisms of Wnt signaling and its implications in developmental biology and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

WIF-1 Antibody (B-10) References:

1. A new secreted protein that binds to Wnt proteins and inhibits their activities.  |  Hsieh, JC., et al. 1999. Nature. 398: 431-6. PMID: 10201374
2. The Wnt antagonist Wif-1 interacts with CTGF and inhibits CTGF activity.  |  Surmann-Schmitt, C., et al. 2012. J Cell Physiol. 227: 2207-16. PMID: 21928342
3. Reduced WIF-1 expression stimulates skin hyperpigmentation in patients with melasma.  |  Kim, JY., et al. 2013. J Invest Dermatol. 133: 191-200. PMID: 22951732
4. Wnt inhibitory factor (WIF)-1 promotes melanogenesis in normal human melanocytes.  |  Park, TJ., et al. 2014. Pigment Cell Melanoma Res. 27: 72-81. PMID: 24131586
5. WIF-1 and Ihh Expression and Clinical Significance in Patients With Lung Squamous Cell Carcinoma and Adenocarcinoma.  |  Zhang, Y. and Hu, C. 2018. Appl Immunohistochem Mol Morphol. 26: 454-461. PMID: 27801732
6. Expression of WIF-1 in inflammatory bowel disease.  |  Terry, R., et al. 2019. Histol Histopathol. 34: 149-157. PMID: 30051904
7. Glucocorticoid therapy suppresses Wnt signaling by reducing the ratio of serum Wnt3a to Wnt inhibitors, sFRP-1 and Wif-1.  |  Kawazoe, M., et al. 2021. Clin Rheumatol. 40: 2947-2954. PMID: 33420868
8. Elevated plasma WIF-1 levels are associated with worse prognosis in heart failure with pulmonary hypertension.  |  Kania, K., et al. 2022. ESC Heart Fail. 9: 4139-4149. PMID: 36082780
9. Dishevelled is a component of the frizzled signaling pathway in Drosophila.  |  Krasnow, RE., et al. 1995. Development. 121: 4095-102. PMID: 8575310
10. Wnt signaling: a common theme in animal development.  |  Cadigan, KM. and Nusse, R. 1997. Genes Dev. 11: 3286-305. PMID: 9407023
11. Dickkopf-1 is a member of a new family of secreted proteins and functions in head induction.  |  Glinka, A., et al. 1998. Nature. 391: 357-62. PMID: 9450748
12. Bridging of beta-catenin and glycogen synthase kinase-3beta by axin and inhibition of beta-catenin-mediated transcription.  |  Sakanaka, C., et al. 1998. Proc Natl Acad Sci U S A. 95: 3020-3. PMID: 9501208

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-WIF-1 Antibody (B-10) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-WIF-1 Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

WIF-1 Antibody (B-10) Specifications

Alternate Product Name: WIF-1

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 331-360

Epitope Target: WIF-1

Epitope Species: human

Product Reactivity/Detection: precursor and mature WIF-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for WIF-1 Antibody (B-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin