Wee 1 Antibody (B-11): sc-5285

Wee 1 Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Wee 1 protein of human origin by WB, IP, IF, IHCP, and ELISA. Wee 1 (B-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, HRP, PE, FITC, and multiple Alexa Fluor® conjugates. Wee 1 protein plays a critical role in cell cycle regulation by acting as a tyrosine-specific protein kinase that phosphorylates Cdc2 on tyrosine 15, which is essential for maintaining Cdc2 in an inactive state during the S and G2 phases of the cell cycle. This regulation prevents premature entry into mitosis, thereby ensuring proper cell division and genomic stability. Additionally, Wee 1 activity is tightly controlled throughout the cell cycle, with function being complemented by Myt 1, another member of the Wee 1 family that phosphorylates Cdc2 on both threonine 14 and tyrosine 15. The interplay between these kinases and the phosphatase Cdc25, which dephosphorylates Cdc2 to activate the Cdc2/cyclin B complex, highlights the intricate balance of phosphorylation and dephosphorylation that governs cell cycle progression.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Wee 1 Antibody (B-11) References:

1. Structure and inhibition of the human cell cycle checkpoint kinase, Wee1A kinase: an atypical tyrosine kinase with a key role in CDK1 regulation.  |  Squire, CJ., et al. 2005. Structure. 13: 541-50. PMID: 15837193
2. Human prostate epithelium lacks Wee1A-mediated DNA damage-induced checkpoint enforcement.  |  Kiviharju-af Hällström, TM., et al. 2007. Proc Natl Acad Sci U S A. 104: 7211-6. PMID: 17431037
3. Human immunodeficiency virus type 1 Vpr binds to the N lobe of the Wee1 kinase domain and enhances kinase activity for CDC2.  |  Kamata, M., et al. 2008. J Virol. 82: 5672-82. PMID: 18385244
4. Wee1(+)-like gene in human cells.  |  Igarashi, M., et al. 1991. Nature. 353: 80-3. PMID: 1840647
5. Differential phosphorylation of vertebrate p34cdc2 kinase at the G1/S and G2/M transitions of the cell cycle: identification of major phosphorylation sites.  |  Krek, W. and Nigg, EA. 1991. EMBO J. 10: 305-16. PMID: 1846803
6. Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis.  |  Morla, AO., et al. 1989. Cell. 58: 193-203. PMID: 2473839
7. A WEE1 family business: regulation of mitosis, cancer progression, and therapeutic target.  |  Ghelli Luserna di Rorà, A., et al. 2020. J Hematol Oncol. 13: 126. PMID: 32958072
8. WEE1 inhibition enhances the antitumor immune response to PD-L1 blockade by the concomitant activation of STING and STAT1 pathways in SCLC.  |  Taniguchi, H., et al. 2022. Cell Rep. 39: 110814. PMID: 35584676
9. SIRT1 deacetylates WEE1 and sensitizes cancer cells to WEE1 inhibition.  |  Zhu, X., et al. 2023. Nat Chem Biol. 19: 585-595. PMID: 36635566
10. Regulation of the human WEE1Hu CDK tyrosine 15-kinase during the cell cycle.  |  Watanabe, N., et al. 1995. EMBO J. 14: 1878-91. PMID: 7743995
11. Human Wee1 kinase inhibits cell division by phosphorylating p34cdc2 exclusively on Tyr15.  |  McGowan, CH. and Russell, P. 1993. EMBO J. 12: 75-85. PMID: 8428596
12. The human Myt1 kinase preferentially phosphorylates Cdc2 on threonine 14 and localizes to the endoplasmic reticulum and Golgi complex.  |  Liu, F., et al. 1997. Mol Cell Biol. 17: 571-83. PMID: 9001210

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Wee 1 Antibody (B-11) has 97 citations in a variety of scientific publications.

Primary antibodies like Anti-Wee 1 Antibody (B-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Wee 1 Antibody (B-11) Specifications

Alternate Product Name: WEE1

Product Clone Name: B-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 347-646

Epitope Target: Wee 1

Epitope Species: human

Product Reactivity/Detection: Wee 1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 97 citations in various scientific publications

Available Conjugates/Forms for Wee 1 Antibody (B-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin