WDR82 Antibody (A-2): sc-518243

WDR82 Antibody (A-2) is a mouse monoclonal IgM antibody specific for an epitope mapping between amino acids 92-112 of WDR82 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). WDR82 monoclonal antibody (A-2) is available as the non-conjugated anti-WDR82 antibody form. WD repeat-containing protein 82 (WDR82), also known as TMEM113, is a 313 amino acid protein characterized by the presence of six WD repeats and classified within the WD repeat SWD2 family. WDR82 (A-2) antibody is primarily localized in the nucleus, where WDR82 serves as a critical component of the Set1 methyltransferase complex, which includes other essential proteins such as Set1A and Set1B. The Set1 complex is responsible for the specific methylation of the Lysine 4 (Lys 4) residue on Histone H3, a modification that is vital for the formation of an active chromatin structure conducive to transcription. WDR82 within this complex is crucial for regulating gene expression, as WDR82 facilitates the chromatin remodeling necessary for transcriptional activation. Given WDR82′s significant role in chromatin modification, WDR82 is essential for maintaining proper cellular function and gene regulation, making anti-WDR82 antibody (A-2) an indispensable tool for studying chromatin dynamics and the regulatory mechanisms underlying gene expression.

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WDR82 Antibody (A-2) References:

1. CpG-binding protein (CXXC finger protein 1) is a component of the mammalian Set1 histone H3-Lys4 methyltransferase complex, the analogue of the yeast Set1/COMPASS complex.  |  Lee, JH. and Skalnik, DG. 2005. J Biol Chem. 280: 41725-31. PMID: 16253997
2. CUL4-DDB1 ubiquitin ligase interacts with multiple WD40-repeat proteins and regulates histone methylation.  |  Higa, LA., et al. 2006. Nat Cell Biol. 8: 1277-83. PMID: 17041588
3. Identification and characterization of the human Set1B histone H3-Lys4 methyltransferase complex.  |  Lee, JH., et al. 2007. J Biol Chem. 282: 13419-28. PMID: 17355966
4. Wdr82 is a C-terminal domain-binding protein that recruits the Setd1A Histone H3-Lys4 methyltransferase complex to transcription start sites of transcribed human genes.  |  Lee, JH. and Skalnik, DG. 2008. Mol Cell Biol. 28: 609-18. PMID: 17998332
5. WDR82, a key epigenetics-related factor, plays a crucial role in normal early embryonic development in mice.  |  Bi, Y., et al. 2011. Biol Reprod. 84: 756-64. PMID: 21123813
6. WDR82 Negatively Regulates Cellular Antiviral Response by Mediating TRAF3 Polyubiquitination in Multiple Cell Lines.  |  Zhu, K., et al. 2015. J Immunol. 195: 5358-66. PMID: 26519536
7. WDR82/PNUTS-PP1 Prevents Transcription-Replication Conflicts by Promoting RNA Polymerase II Degradation on Chromatin.  |  Landsverk, HB., et al. 2020. Cell Rep. 33: 108469. PMID: 33264625
8. WDR82-binding long noncoding RNA lncEry controls mouse erythroid differentiation and maturation.  |  Yang, S., et al. 2022. J Exp Med. 219: PMID: 35315911
9. Molecular insight into the SETD1A/B N-terminal region and its interaction with WDR82.  |  Bao, S. and Xu, C. 2023. Biochem Biophys Res Commun. 658: 136-140. PMID: 37030068
10. A restrictor complex of ZC3H4, WDR82, and ARS2 integrates with PNUTS to control unproductive transcription.  |  Estell, C., et al. 2023. Mol Cell. 83: 2222-2239.e5. PMID: 37329883
11. WDR82-Mediated H3K4me3 Is Associated with Tumor Proliferation and Therapeutic Efficacy in Pediatric High-Grade Gliomas.  |  Wadhwani, N., et al. 2023. Cancers (Basel). 15: PMID: 37444539
12. Premature transcription termination complex proteins PCF11 and WDR82 silence HIV-1 expression in latently infected cells.  |  Ait Said, M., et al. 2023. Proc Natl Acad Sci U S A. 120: e2313356120. PMID: 38015843

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-WDR82 Antibody (A-2) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-WDR82 Antibody (A-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

WDR82 Antibody (A-2) Specifications

Alternate Product Name: WDR82

Product Clone Name: A-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 92-112

Epitope Target: WDR82

Epitope Species: human

Product Reactivity/Detection: WDR82

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for WDR82 Antibody (A-2): N/A