WDR48 Antibody (E-4): sc-514473
- WDR48 Antibody (E-4) is a mouse monoclonal IgG2a κ WDR48 antibody, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 530-640 mapping near the C-terminus of WDR48 of human origin
- WDR48 Antibody (E-4) is recommended for detection of WDR48 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-WDR48 Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for WDR48 Antibody (E-4) for WB applications. These reagents are now offered in bundles with WDR48 Antibody (E-4) (see ordering information below).
WDR48 Antibody (E-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects WDR48 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). WDR48 (E-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. WDR48, also known as USP1-associated factor 1 and p80, is a 677 amino acid protein characterized by eight WD repeats, which are motifs that typically form a tertiary propeller structure. These WD repeats are crucial for WDR48′s function, as they facilitate protein-protein interactions and the assembly of protein complexes, thereby playing a significant role in regulatory mechanisms such as signal transduction, apoptosis, transcriptional regulation, and cell cycle control. WDR48 is essential for regulating deubiquitinating complexes by activating USP1, USP12, and USP46, enhancing deubiquitination through increased catalytic turnover without altering the affinity of deubiquitinating enzymes for their substrates. WDR48 is ubiquitously expressed and primarily localized in the cytoplasm, with five isoforms resulting from alternative splicing events, highlighting its versatility and importance in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
WDR48 Antibody (E-4) References:
- The WD repeat: a common architecture for diverse functions. | Smith, TF., et al. 1999. Trends Biochem Sci. 24: 181-5. PMID: 10322433
- Thirty-plus functional families from a single motif. | Yu, L., et al. 2000. Protein Sci. 9: 2470-6. PMID: 11206068
- Herpesviral protein targets a cellular WD repeat endosomal protein to downregulate T lymphocyte receptor expression. | Park, J., et al. 2002. Immunity. 17: 221-33. PMID: 12196293
- Distinct roles of cellular Lck and p80 proteins in herpesvirus saimiri Tip function on lipid rafts. | Park, J., et al. 2003. J Virol. 77: 9041-51. PMID: 12885920
- Human papillomavirus E1 helicase interacts with the WD repeat protein p80 to promote maintenance of the viral genome in keratinocytes. | Côté-Martin, A., et al. 2008. J Virol. 82: 1271-83. PMID: 18032488
- A UAF1-containing multisubunit protein complex regulates the Fanconi anemia pathway. | Cohn, MA., et al. 2007. Mol Cell. 28: 786-97. PMID: 18082604
- UAF1 is a subunit of multiple deubiquitinating enzyme complexes. | Cohn, MA., et al. 2009. J Biol Chem. 284: 5343-51. PMID: 19075014
- The ancient regulatory-protein family of WD-repeat proteins. | Neer, EJ., et al. 1994. Nature. 371: 297-300. PMID: 8090199
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WDR48 Antibody (E-4) | sc-514473 | 200 µg/ml | $322.00 | |||
WDR48 Antibody (E-4): m-IgG Fc BP-HRP Bundle | sc-531142 | 200 µg Ab; 10 µg BP | $361.00 | |||
WDR48 Antibody (E-4): m-IgGκ BP-HRP Bundle | sc-524742 | 200 µg Ab, 40 µg BP | $361.00 | |||
WDR48 Antibody (E-4): m-IgG2a BP-HRP Bundle | sc-547701 | 200 µg Ab; 10 µg BP | $361.00 | |||
WDR48 Antibody (E-4) AC | sc-514473 AC | 500 µg/ml, 25% agarose | $424.00 | |||
WDR48 Antibody (E-4) HRP | sc-514473 HRP | 200 µg/ml | $322.00 | |||
WDR48 Antibody (E-4) FITC | sc-514473 FITC | 200 µg/ml | $336.00 | |||
WDR48 Antibody (E-4) PE | sc-514473 PE | 200 µg/ml | $349.00 | |||
WDR48 Antibody (E-4) Alexa Fluor® 488 | sc-514473 AF488 | 200 µg/ml | $364.00 | |||
WDR48 Antibody (E-4) Alexa Fluor® 546 | sc-514473 AF546 | 200 µg/ml | $364.00 | |||
WDR48 Antibody (E-4) Alexa Fluor® 594 | sc-514473 AF594 | 200 µg/ml | $364.00 | |||
WDR48 Antibody (E-4) Alexa Fluor® 647 | sc-514473 AF647 | 200 µg/ml | $364.00 | |||
WDR48 Antibody (E-4) Alexa Fluor® 680 | sc-514473 AF680 | 200 µg/ml | $364.00 | |||
WDR48 Antibody (E-4) Alexa Fluor® 790 | sc-514473 AF790 | 200 µg/ml | $364.00 |