WAVE Antibody (F-10): sc-365165

WAVE Antibody (F-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects WAVE protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-WAVE antibody (F-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. WAVE, also known as WASP Family Member 1 and SCAR (Suppressor of Cell Agitation Response), plays a crucial role in actin polymerization and cytoskeletal organization, which are essential for various cellular processes such as cell motility, shape, and signaling. WAVE1, a member of the WAVE family, is primarily expressed in the brain and regulates membrane ruffling by inducing actin filament cluster formation in response to GTP binding and activating Rac. This regulation is vital for dynamic reorganization of the actin cytoskeleton, necessary for processes like cell migration and cellular structure formation. WAVE proteins interact with the Arp2/3 complex to promote actin filament formation, facilitating cellular responses to external stimuli. WAVE presence in various tissues underscores importance in both normal physiological functions and potential pathological conditions, making anti-WAVE antibody (F-10) a valuable tool for researchers studying actin dynamics and related cellular mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

WAVE Antibody (F-10) References:

1. Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp2/3 complex.  |  Machesky, LM., et al. 1999. Proc Natl Acad Sci U S A. 96: 3739-44. PMID: 10097107
2. The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly.  |  Rohatgi, R., et al. 1999. Cell. 97: 221-31. PMID: 10219243
3. Structure of the enabled/VASP homology 1 domain-peptide complex: a key component in the spatial control of actin assembly.  |  Prehoda, KE., et al. 1999. Cell. 97: 471-80. PMID: 10338211
4. Identification of two human WAVE/SCAR homologues as general actin regulatory molecules which associate with the Arp2/3 complex.  |  Suetsugu, S., et al. 1999. Biochem Biophys Res Commun. 260: 296-302. PMID: 10381382
5. The next wave: protein tyrosine phosphatases enter T cell antigen receptor signalling.  |  Mustelin, T., et al. 1999. Cell Signal. 11: 637-50. PMID: 10530872
6. Role for the Abi/wave protein complex in T cell receptor-mediated proliferation and cytoskeletal remodeling.  |  Zipfel, PA., et al. 2006. Curr Biol. 16: 35-46. PMID: 16401422
7. The WASP-WAVE protein network: connecting the membrane to the cytoskeleton.  |  Takenawa, T. and Suetsugu, S. 2007. Nat Rev Mol Cell Biol. 8: 37-48. PMID: 17183359
8. WASP-1, a canonical Wnt signaling potentiator, rescues hippocampal synaptic impairments induced by Aβ oligomers.  |  Vargas, JY., et al. 2015. Exp Neurol. 264: 14-25. PMID: 25450465
9. Wiskott-Aldrich syndrome protein, a novel effector for the GTPase CDC42Hs, is implicated in actin polymerization.  |  Symons, M., et al. 1996. Cell. 84: 723-34. PMID: 8625410
10. SCAR, a WASP-related protein, isolated as a suppressor of receptor defects in late Dictyostelium development.  |  Bear, JE., et al. 1998. J Cell Biol. 142: 1325-35. PMID: 9732292
11. WAVE, a novel WASP-family protein involved in actin reorganization induced by Rac.  |  Miki, H., et al. 1998. EMBO J. 17: 6932-41. PMID: 9843499
12. Scar1 and the related Wiskott-Aldrich syndrome protein, WASP, regulate the actin cytoskeleton through the Arp2/3 complex.  |  Machesky, LM. and Insall, RH. Curr Biol. 8: 1347-56. PMID: 9889097

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-WAVE Antibody (F-10) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-WAVE Antibody (F-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

WAVE Antibody (F-10) Specifications

Alternate Product Name: WASF1

Product Clone Name: F-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-180

Epitope Target: WAVE

Epitope Species: human

Product Reactivity/Detection: WAVE1, WAVE2 and WAVE3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for WAVE Antibody (F-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred WAVE2 antibody is WAVE2 Antibody (C-6): sc-373889.