Anti-VPS25 Antibody (B-4) recommended for detection of VPS25 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-VPS25 Antibody (B-4): sc-271648

Anti-VPS25 Antibody (B-4) is rated 5.0 out of 5 by 6.
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Datasheets

    • Anti-VPS25 Antibody (B-4) is a mouse monoclonal IgG1 κ VPS25 antibody, cited in 5 publications, provided at 200 µg/ml
    • raised against amino acids 1-176 representing full length VPS25 of human origin
    • Anti-VPS25 Antibody (B-4) is recommended for detection of VPS25 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-VPS25 Antibody (B-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of VPS25 (B-4): sc-271648.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for VPS25 Antibody (B-4) for WB and IHC(P) applications. This reagent is now offered in a bundle with VPS25 Antibody (B-4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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I am using VPS25 (B-4): sc-271648 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Worked well for Western Blot. I used this anti-VPS25 for a Western Blot, with m-IgGκ BP-HRP, and it showed specific bands for Vps25 in HeLa whole cell lysates and post-nuclear supernatant fractions.
Date published: 2017-11-30
Rated 5 out of 5 by from Good antibody I used this antibody recently. It works well and less background.
Date published: 2017-09-12
Rated 5 out of 5 by from Reliable antibody Detects a consistent and strong band at the correct molecular weight of ~21 kDa in Western Blot.
Date published: 2017-02-07
Rated 5 out of 5 by from Robust signal in WB in NIH/3T3 whole cell Robust signal in WB in NIH/3T3 whole cell lysate and HeLa nuclear extract. -SCBT QC
Date published: 2015-05-15
Rated 5 out of 5 by from WB publishable data with human HuH WB publishable data with human HuH-7 - SCBT Publication Review
Date published: 2015-03-23
Rated 5 out of 5 by from Produced beautiful immunoperoxidase nuclear Produced beautiful immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human nasopharynx tissue. -SCBT QC
Date published: 2013-12-11
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