VPS16 Antibody (2F10): sc-293327
- VPS16 Antibody (2F10) is a mouse monoclonal IgG2a κ VPS16 antibody provided at 100 µg/ml
- raised against amino acids 754-839 of VPS16 of human origin
- recommended for detection of VPS16 of human origin by WB, IP, IF and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for VPS16 Antibody (2F10). This work is in progress.
VPS16 Antibody (2F10) is a mouse monoclonal IgG2a kappa light chain antibody that detects the VPS16 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-VPS16 antibody (2F10) is available as a non-conjugated monoclonal isotype antibody. VPS16, or vacuolar protein sorting 16, is a crucial component of the Class C VPS protein complex, which includes VPS11, VPS18, and VPS33, and is essential for the proper trafficking of endocytic and biosynthetic proteins to the vacuole. This 839 amino acid protein is predominantly located on the cytoplasmic side of membranes and is ubiquitously expressed across various tissues. VPS16′s role in membrane docking and fusion reactions of late endosomes and lysosomes is vital for maintaining cellular homeostasis, as VPS16 facilitates vesicle-mediated protein trafficking to lysosomal compartments. Disruptions in VPS16 function due to genetic mutations can lead to impaired lysosomal trafficking, contributing to diseases such as Hermansky-Pudlak syndrome. VPS16 (2F10) antibody′s ability to effectively detect VPS16 makes this reagent an invaluable tool for researchers studying lysosomal function and related pathologies.
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VPS16 Antibody (2F10) References:
- Class C Vps protein complex regulates vacuolar SNARE pairing and is required for vesicle docking/fusion. | Sato, TK., et al. 2000. Mol Cell. 6: 661-71. PMID: 11030345
- Molecular cloning and characterization of human VPS18, VPS 11, VPS16, and VPS33. | Huizing, M., et al. 2001. Gene. 264: 241-7. PMID: 11250079
- Identification of mouse Vps16 and biochemical characterization of mammalian class C Vps complex. | Kim, BY., et al. 2003. Biochem Biophys Res Commun. 311: 577-82. PMID: 14623309
- Expression of the mammalian homologue of vacuolar protein sorting 16 (Vps16p) in the mouse and rat brain. | Kim, BY., et al. 2004. Neurosci Lett. 355: 217-20. PMID: 14732470
- Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules. | Pulipparacharuvil, S., et al. 2005. J Cell Sci. 118: 3663-73. PMID: 16046475
- The VPS16 gene product associates with a sedimentable protein complex and is essential for vacuolar protein sorting in yeast. | Horazdovsky, BF. and Emr, SD. 1993. J Biol Chem. 268: 4953-62. PMID: 8444873
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VPS16 Antibody (2F10) | sc-293327 | 100 µg/ml | $322.00 |