VpreB Antibody (H-11): sc-514957

VpreB Antibody (H-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects VpreB protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). VpreB (H-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. VpreB protein, also known as CD179a or pre-B lymphocyte 1, plays a crucial role in B cell development, specifically in pre-B lymphocytes, where VpreB is expressed but absent in mature B cells and other blood cell lineages. VpreB protein is essential for forming a molecular complex with the Ig-mu chain, which regulates immunoglobulin gene rearrangements during early B cell differentiation. The gene encoding VpreB is located on human chromosome 22q11.2, and in mice, VpreB and lambda 5 genes are co-expressed in pre-B cells as part of the same transcription unit, allowing for production of a primary transcript that undergoes alternative splicing to yield pre-B and lambda 5 mRNAs. In humans, these genes are separate entities, highlighting evolutionary differences in B cell development between species. The unique expression pattern and functional significance of VpreB make anti-VpreB antibody (H-11) an invaluable tool for studying B cell biology and related immunological processes.

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VpreB Antibody (H-11) References:

1. The VpreB protein of the surrogate light-chain can pair with some mu heavy-chains in the absence of the lambda 5 protein.  |  Seidl, T., et al. 2001. Eur J Immunol. 31: 1999-2006. PMID: 11449352
2. Analysis of VpreB expression during B lineage differentiation in lambda5-deficient mice.  |  Stephan, RP., et al. 2001. J Immunol. 167: 3734-9. PMID: 11564789
3. The VpreB1 enhancer drives developmental stage-specific gene expression in vivo.  |  Licence, S., et al. 2003. Eur J Immunol. 33: 1117-26. PMID: 12672078
4. Variable domains and a VpreB-like molecule are present in a jawless vertebrate.  |  Cannon, JP., et al. 2005. Immunogenetics. 56: 924-9. PMID: 15650874
5. The pre-B-cell receptor induces silencing of VpreB and lambda5 transcription.  |  Parker, MJ., et al. 2005. EMBO J. 24: 3895-905. PMID: 16281060
6. The human pre-B-specific lambda-like cluster is located in the 22q11.2-22q12.3 region, distal to the IgC lambda locus.  |  Mattei, MG., et al. 1991. Genomics. 9: 544-6. PMID: 1903358
7. A second gene, VpreB in the lambda 5 locus of the mouse, which appears to be selectively expressed in pre-B lymphocytes.  |  Kudo, A. and Melchers, F. 1987. EMBO J. 6: 2267-72. PMID: 3117530
8. The omega and iota surrogate immunoglobulin light chains.  |  Pillai, S. and Baltimore, D. 1988. Curr Top Microbiol Immunol. 137: 136-9. PMID: 3138073
9. The human Vpre B gene is located on chromosome 22 near a cluster of V lambda gene segments.  |  Bauer, SR., et al. 1988. Immunogenetics. 28: 328-33. PMID: 3139558

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-VpreB Antibody (H-11) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-VpreB Antibody (H-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

VpreB Antibody (H-11) Specifications

Alternate Product Name: VpreB

Product Clone Name: H-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 91-145

Epitope Target: VpreB

Epitope Species: human

Product Reactivity/Detection: VpreB

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for VpreB Antibody (H-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin