VP5 Antibody (6F10): sc-13525
- VP5 Antibody (6F10) is a mouse monoclonal IgG1 κ VP5 antibody, cited in 15 publications, provided at 200 µg/ml
- raised against amino acids 862-880 of VP5 from HSV-1
- VP5 Antibody (6F10) is recommended for detection of VP5 and VP5 fusion proteins by WB, IP and IF
- Anti-VP5 Antibody (6F10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for VP5 Antibody (6F10) for WB applications. These reagents are now offered in bundles with VP5 Antibody (6F10) (see ordering information below).
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VP5 Antibody (6F10) is a mouse monoclonal IgG1 kappa light chain antibody that detects VP5 protein by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). VP5 (6F10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. VP5 protein plays a crucial role in the structural integrity of the herpes simplex virus type 1 (HSV-1) capsid, which is essential for protecting double-stranded DNA and facilitating infection. Located within the infected-cell nucleus, VP5 is the predominant component of the capsomer, forming hexons and pentons that create the intricate icosahedral structure of the viral capsid. VP5 hexons interact with scaffolding protein VP26, while VP5 pentons are strategically positioned at the vertices of the capsid. This precise arrangement ensures proper encapsulation of the viral genome and aids in efficient delivery of viral particles during infection.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
VP5 Antibody (6F10) References:
- Assembly of the herpes simplex virus procapsid from purified components and identification of small complexes containing the major capsid and scaffolding proteins. | Newcomb, WW., et al. 1999. J Virol. 73: 4239-50. PMID: 10196320
- Roles of triplex and scaffolding proteins in herpes simplex virus type 1 capsid formation suggested by structures of recombinant particles. | Saad, A., et al. 1999. J Virol. 73: 6821-30. PMID: 10400780
- Structure of the herpes simplex virus capsid: effects of extraction with guanidine hydrochloride and partial reconstitution of extracted capsids. | Newcomb, WW. and Brown, JC. 1991. J Virol. 65: 613-20. PMID: 1846187
- Hexon-only binding of VP26 reflects differences between the hexon and penton conformations of VP5, the major capsid protein of herpes simplex virus. | Wingfield, PT., et al. 1997. J Virol. 71: 8955-61. PMID: 9371551
- Identification of the sites of interaction between the scaffold and outer shell in herpes simplex virus-1 capsids by difference electron imaging. | Zhou, ZH., et al. 1998. Proc Natl Acad Sci U S A. 95: 2778-83. PMID: 9501166
- Assembly of the herpes simplex virus capsid: preformed triplexes bind to the nascent capsid. | Spencer, JV., et al. 1998. J Virol. 72: 3944-51. PMID: 9557680
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VP5 Antibody (6F10) | sc-13525 | 200 µg/ml | $316.00 | |||
VP5 Antibody (6F10): m-IgG Fc BP-HRP Bundle | sc-526552 | 200 µg Ab; 10 µg BP | $354.00 | |||
VP5 Antibody (6F10): m-IgG1 BP-HRP Bundle | sc-531925 | 200 µg Ab; 20 µg BP | $354.00 | |||
VP5 Antibody (6F10) AC | sc-13525 AC | 500 µg/ml, 25% agarose | $416.00 | |||
VP5 Antibody (6F10) HRP | sc-13525 HRP | 200 µg/ml | $316.00 | |||
VP5 Antibody (6F10) FITC | sc-13525 FITC | 200 µg/ml | $330.00 | |||
VP5 Antibody (6F10) PE | sc-13525 PE | 200 µg/ml | $343.00 | |||
VP5 Antibody (6F10) Alexa Fluor® 488 | sc-13525 AF488 | 200 µg/ml | $357.00 | |||
VP5 Antibody (6F10) Alexa Fluor® 546 | sc-13525 AF546 | 200 µg/ml | $357.00 | |||
VP5 Antibody (6F10) Alexa Fluor® 594 | sc-13525 AF594 | 200 µg/ml | $357.00 | |||
VP5 Antibody (6F10) Alexa Fluor® 647 | sc-13525 AF647 | 200 µg/ml | $357.00 | |||
VP5 Antibody (6F10) Alexa Fluor® 680 | sc-13525 AF680 | 200 µg/ml | $357.00 | |||
VP5 Antibody (6F10) Alexa Fluor® 790 | sc-13525 AF790 | 200 µg/ml | $357.00 |