Vitronectin 65/75 Antibody (D-8): sc-74484

Vitronectin 65/75 Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Vitronectin 65/75 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Vitronectin 65/75 (D-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Vitronectin plays a crucial role in the extracellular matrix, where Vitronectin is involved in cell adhesion, migration, and wound healing processes. Vitronectin is characterized by an RGD (Arg-Gly-Asp) sequence, which is vital for binding to integrins on cell surfaces, facilitating cell attachment and spreading. This interaction is particularly important in tissue repair, as increased levels of Vitronectin have been observed in migrating cells during wound healing. Vitronectin′s ability to bind to plasminogen activators and integrins underscores Vitronectin′s significance in regulating cell motility and extracellular matrix dynamics. Post-translational modifications of Vitronectin, such as glycosylation, can influence interactions with other proteins, thereby impacting cellular responses during injury and repair.

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Vitronectin 65/75 Antibody (D-8) References:

1. Motility of fibronectin receptor-deficient cells on fibronectin and vitronectin: collaborative interactions among integrins.  |  Bauer, JS., et al. 1992. J Cell Biol. 116: 477-87. PMID: 1370495
2. Heparin-binding properties of vitronectin are linked to complex formation as illustrated by in vitro polymerization and binding to the terminal complement complex.  |  Høgåsen, K., et al. 1992. J Biol Chem. 267: 23076-82. PMID: 1385412
3. Vitronectin and collagen I differentially regulate osteogenesis in mesenchymal stem cells.  |  Kundu, AK. and Putnam, AJ. 2006. Biochem Biophys Res Commun. 347: 347-57. PMID: 16815299
4. Presence of plasma complement regulatory proteins clusterin (Apo J) and vitronectin (S40) on circulating immune complexes (CIC).  |  Chauhan, AK. and Moore, TL. 2006. Clin Exp Immunol. 145: 398-406. PMID: 16907906
5. Plasmin cleavage of vitronectin. Identification of the site and consequent attenuation in binding plasminogen activator inhibitor-1.  |  Chain, D., et al. 1991. FEBS Lett. 285: 251-6. PMID: 1713175
6. Cyclic AMP-dependent protein kinase phosphorylates serine378 in vitronectin.  |  Mehringer, JH., et al. 1991. Biochem Biophys Res Commun. 179: 655-60. PMID: 1715701
7. Role of vitronectin-like protein in Agrobacterium attachment and transformation of Arabidopsis cells.  |  Clauce-Coupel, H., et al. 2008. Protoplasma. 234: 65-75. PMID: 18841324
8. The structure of fibronectin and its role in cellular adhesion.  |  Akiyama, SK., et al. 1981. J Supramol Struct Cell Biochem. 16: 345-8. PMID: 6273596
9. Molecular and biological interactions of fibronectin.  |  Ruoslahti, E., et al. 1982. J Invest Dermatol. 79 Suppl 1: 65s-68s. PMID: 7045246
10. Site-directed mutagenesis of the arginine-glycine-aspartic acid in vitronectin abolishes cell adhesion.  |  Cherny, RC., et al. 1993. J Biol Chem. 268: 9725-9. PMID: 7683657
11. The serpin PAI-1 inhibits cell migration by blocking integrin alpha V beta 3 binding to vitronectin.  |  Stefansson, S. and Lawrence, DA. 1996. Nature. 383: 441-3. PMID: 8837777
12. Differential modulation of cell adhesion by interaction between adhesive and counter-adhesive proteins: characterization of the binding of vitronectin to osteonectin (BM40, SPARC).  |  Rosenblatt, S., et al. 1997. Biochem J. 324 (Pt 1): 311-9. PMID: 9164872

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Vitronectin 65/75 Antibody (D-8) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-Vitronectin 65/75 Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Vitronectin 65/75 Antibody (D-8) Specifications

Alternate Product Name: Vitronectin

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-270

Epitope Target: Vitronectin 75

Epitope Species: human

Product Reactivity/Detection: Vitronectin 65 and Vitronectin 75

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for Vitronectin 65/75 Antibody (D-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin