Date published: 2026-5-7

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vinculin Antibody (V284): sc-59803

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Datasheets
  • vinculin Antibody (V284) is a mouse monoclonal IgG1 κ, cited in 25 publications, provided at 200 µg/ml
  • raised against vinculin from platelets of human origin
  • recommended for detection of vinculin of mouse, rat and human origin by WB, IP, IF and IHC(P)
  • See vinculin (7F9): sc-73614 for vinculin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for vinculin Antibody (V284) for WB and IHC(P) applications. These reagents are now offered in bundles with vinculin Antibody (V284) (see ordering information below).
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Vinculin Antibody (V284) is a mouse monoclonal IgG1 antibody that detects vinculin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-vinculin antibody (V284) plays a crucial role in cellular adhesion and signaling by linking the actin cytoskeleton to integrins at focal adhesions, which are essential for maintaining cell shape and facilitating communication with the extracellular matrix. Vinculin is primarily located at the plasma membrane, where vinculin forms complexes with other cytoskeletal proteins, such as talin and α-actinin, to stabilize cell adhesion and promote cellular responses to mechanical stress. Proper functioning of vinculin is vital for processes like cell migration, tissue repair, and the overall integrity of tissues, making vinculin a significant focus of study in various fields, including cancer research and developmental biology. Anti-vinculin antibody (V284) serves as an invaluable tool for researchers aiming to explore the dynamics of cell adhesion and the intricate signaling pathways that govern cellular behavior.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

vinculin Antibody (V284)

sc-59803
200 µg/ml
$322.00

vinculin Antibody (V284): m-IgG Fc BP-HRP Bundle

sc-539140
200 µg Ab; 10 µg BP
$361.00

vinculin Antibody (V284): m-IgG1 BP-HRP Bundle

sc-541288
200 µg Ab; 20 µg BP
$361.00

I am using vinculin (V284): sc-59803 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Anti-vinculin works well for ICCAnti-vinculin works well for ICC on mammary epithelial cells.
Date published: 2024-03-21
Rated 5 out of 5 by from recommended for use in IHC(P)Beautiful cytoplasmic immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue, human bone marrow tissue and human testis tissue.
Date published: 2017-01-31
Rated 4 out of 5 by from Positive results in western blot analysisPositive results in western blot analysis of vinculin expression in HeLa, K-562, U-937 and PC-3 whole cell lysates. -SCBT QC
Date published: 2015-05-13
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmicProduced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human smooth muscle tissue. -SCBT QC
Date published: 2015-03-14
Rated 5 out of 5 by from Published data using V284Published data using V284 - cell culture with PC-3 cells. -SCBT Publication Review
Date published: 2015-01-12
Rated 5 out of 5 by from Nice data in WB with nonNice data in WB with non-transfected 293T, human vinculin transfected 293T and HUV-EC-C whole cell lysates. -SCBT QC
Date published: 2015-01-09
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vinculin Antibody (V284) is rated 4.8 out of 5 by 6.
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  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_en_US, sid_59803, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
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