vinculin Antibody (H-10): sc-25336
- vinculin Antibody (H-10) is a mouse monoclonal IgG2a κ, cited in 197 publications, provided at 200 µg/ml
- raised against amino acids 1-300 of vinculin of human origin
- Anti-vinculin Antibody (H-10) is recommended for detection of vinculin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-vinculin Antibody (H-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for vinculin Antibody (H-10) for WB and IHC(P) applications. These reagents are now offered in bundles with vinculin Antibody (H-10) (see ordering information below).
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SEE ALSO...
Vinculin Antibody (H-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects vinculin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-vinculin antibody (H-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Vinculin plays a crucial role in cellular adhesion and mechanotransduction, primarily located at focal adhesions, which are specialized structures that connect the extracellular matrix to the actin cytoskeleton. These focal adhesions are essential for maintaining cell shape, motility, and signaling, as they facilitate the transmission of mechanical forces and biochemical signals between the cell and its environment. The interaction of vinculin with other cytoskeletal proteins, such as Talin and α-actinin, is vital for the assembly and stability of these adhesion complexes. Furthermore, vinculin′s ability to bind to actin and other focal adhesion proteins underscores its importance in regulating cellular responses to mechanical stress and promoting cell migration, making vinculin a key player in processes such as wound healing and tissue repair.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
vinculin Antibody (H-10) | sc-25336 | 200 µg/ml | $322.00 | |||
vinculin Antibody (H-10): m-IgG Fc BP-HRP Bundle | sc-525517 | 200 µg Ab; 10 µg BP | $361.00 | |||
vinculin Antibody (H-10): m-IgG2a BP-HRP Bundle | sc-546300 | 200 µg Ab; 10 µg BP | $361.00 | |||
vinculin Antibody (H-10) AC | sc-25336 AC | 500 µg/ml, 25% agarose | $424.00 | |||
vinculin Antibody (H-10) HRP | sc-25336 HRP | 200 µg/ml | $322.00 | |||
vinculin Antibody (H-10) FITC | sc-25336 FITC | 200 µg/ml | $336.00 | |||
vinculin Antibody (H-10) PE | sc-25336 PE | 200 µg/ml | $349.00 | |||
vinculin Antibody (H-10) Alexa Fluor® 488 | sc-25336 AF488 | 200 µg/ml | $364.00 | |||
vinculin Antibody (H-10) Alexa Fluor® 546 | sc-25336 AF546 | 200 µg/ml | $364.00 | |||
vinculin Antibody (H-10) Alexa Fluor® 594 | sc-25336 AF594 | 200 µg/ml | $364.00 | |||
vinculin Antibody (H-10) Alexa Fluor® 647 | sc-25336 AF647 | 200 µg/ml | $364.00 | |||
vinculin Antibody (H-10) Alexa Fluor® 680 | sc-25336 AF680 | 200 µg/ml | $364.00 | |||
vinculin Antibody (H-10) Alexa Fluor® 790 | sc-25336 AF790 | 200 µg/ml | $364.00 |