vinculin Antibody (G-11): sc-55465

Vinculin Antibody (G-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects vinculin from mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-vinculin antibody (G-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Vinculin plays a crucial role in cellular adhesion and mechanotransduction, functioning as a key component of focal adhesions, which are specialized structures that connect the cytoskeleton to the extracellular matrix. These focal adhesions are essential for maintaining cell shape, enabling cell migration, and facilitating communication between the cell and its environment. Vinculin′s ability to interact with other cytoskeletal proteins, such as Talin and α-actinin, underscores vinculin′s importance in linking the actin cytoskeleton to integrins in the plasma membrane, thereby influencing cellular responses to mechanical stimuli. Expression levels of vinculin and Talin are closely regulated by the availability of actin, highlighting the intricate balance of these proteins in cellular dynamics. With versatile applications and critical role in cell biology, vinculin monoclonal antibody (G-11) serves as an invaluable tool for researchers studying cell adhesion and signaling pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-vinculin Antibody (G-11) has 37 citations in a variety of scientific publications.

Primary antibodies like Anti-vinculin Antibody (G-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

vinculin Antibody (G-11) Specifications

Alternate Product Name: vinculin

Product Clone Name: G-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300

Epitope Target: vinculin

Epitope Species: human

Product Reactivity/Detection: vinculin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 37 citations in various scientific publications

Available Conjugates/Forms for vinculin Antibody (G-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred vinculin antibody is vinculin Antibody (7F9): sc-73614.