Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Vigilin CRISPR/Cas9 KO Plasmid (m): sc-431035
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Vigilin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Vigilin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Vigilin Antibody (H-3): sc-271523
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Vigilin CRISPR/Cas9 KO Plasmid (m) | sc-431035 | 20 µg | $397.00 |
Overview
Mouse Hdlbp encodes vigilin, a large RNA-binding protein with multiple KH domains that associates with mRNA and ribonucleoprotein complexes to influence transcript stability, localization, and translation. Vigilin has been linked to regulation of lipid and sterol metabolic gene expression through interactions with specific mRNAs, connecting it to broader pathways controlling cellular energy balance and membrane homeostasis. It also participates in post-transcriptional gene control during proliferation and stress responses, consistent with roles in RNA processing and translational regulation. Dysregulation of vigilin-associated RNA networks has been investigated in contexts involving metabolic phenotypes and altered gene-expression programs relevant to disease mechanisms.
Vigilin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hdlbp gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hdlbp together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hdlbp open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Vigilin protein expression.
This CRISPR knockout system enables efficient generation of Hdlbp-deficient cell models for investigation of Vigilin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hdlbp exon(s) critical for Vigilin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hdlbp genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Vigilin CRISPR/Cas9 KO Plasmid (m) and Vigilin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hdlbp locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Vigilin HDR Plasmid (m) and Vigilin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hdlbp homology arms to support homology-directed repair at defined Hdlbp target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.