Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
vezatin CRISPR/Cas9 KO Plasmid (h): sc-418454
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- vezatin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the vezatin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: vezatin Antibody (B-1): sc-271347
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
vezatin CRISPR/Cas9 KO Plasmid (h) | sc-418454 | 20 µg | $397.00 |
Overview
VEZT encodes vezatin, a transmembrane cell junction protein that associates with the cadherin–catenin complex and links adherens junctions to the actin cytoskeleton. Vezatin contributes to epithelial cell–cell adhesion, junctional organization, and mechanotransduction processes that influence tissue architecture and polarity. Through its role at adherens junctions, VEZT intersects with pathways governing cytoskeletal dynamics and contact-dependent signaling. Altered VEZT expression or junctional remodeling has been investigated in contexts of epithelial dysfunction and cancer-related changes in adhesion and invasiveness.
vezatin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the VEZT gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the VEZT together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the VEZT open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish vezatin protein expression.
This CRISPR knockout system enables efficient generation of VEZT-deficient cell models for investigation of vezatin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting VEZT exon(s) critical for vezatin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple VEZT genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by vezatin CRISPR/Cas9 KO Plasmid (h) and vezatin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the VEZT locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by vezatin HDR Plasmid (h) and vezatin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by VEZT homology arms to support homology-directed repair at defined VEZT target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.