Date published: 2026-4-12

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Flk-1/KDR/VEGFR2 Antibody (D-8): sc-393163

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Datasheets
  • Flk-1/KDR/VEGFR2 Antibody (D-8) is a mouse monoclonal IgG1 λ Flk-1/KDR/VEGFR2 antibody, cited in 32 publications, provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 1282-1321 at the C-terminus of VEGFR2 of human origin
  • Flk-1/KDR/VEGFR2 Antibody (D-8) is recommended for detection of VEGFR2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
  • Anti-Flk-1/KDR/VEGFR2 Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for Flk-1/KDR/VEGFR2 Antibody (D-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with Flk-1/KDR/VEGFR2 Antibody (D-8) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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VEGFR2 Antibody (D-8) is a mouse monoclonal IgG1 lambda light chain antibody designed to detect Vascular Endothelial Growth Factor Receptor 2 (VEGFR2), also known as KDR in humans and Flk-1 in mice. Anti-VEGFR2 antibody (D-8) targets a specific epitope within amino acids 1282-1321 at the C-terminus of human VEGFR2, ensuring reliable binding across multiple species including mouse, rat, human, equine, canine, bovine, and porcine. VEGFR2 is a critical receptor tyrosine kinase located on the cell surface of endothelial cells, where it plays a vital role in angiogenesis by mediating the effects of VEGF ligands. This regulation of endothelial cell proliferation, migration, and survival is essential for normal physiological processes such as embryonic development and wound healing, as well as in pathological conditions like cancer, where angiogenesis facilitates tumor growth and metastasis. VEGFR2 monoclonal antibody (D-8) is versatile in its applications, suitable for western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA), thereby supporting a wide range of research and diagnostic studies. Anti-VEGFR2 antibody (D-8) is available in both non-conjugated forms and with multiple conjugates, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and Alexa Fluor® variants, providing flexibility to meet diverse experimental needs. VEGFR2 (D-8) antibody is recommended for scientists exploring the molecular mechanisms underlying angiogenesis and who are developing targeted therapeutic strategies aimed at regulating blood vessel formation in various disease contexts.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Flk-1/KDR/VEGFR2 Antibody (D-8)

sc-393163
200 µg/ml
$322.00

Flk-1/KDR/VEGFR2 Antibody (D-8): m-IgGλ BP-HRP Bundle

sc-523832
200 µg Ab, 40 µg BP
$361.00

Flk-1/KDR/VEGFR2 Antibody (D-8) AC

sc-393163 AC
500 µg/ml, 25% agarose
$424.00

Flk-1/KDR/VEGFR2 Antibody (D-8) HRP

sc-393163 HRP
200 µg/ml
$322.00

Flk-1/KDR/VEGFR2 Antibody (D-8) FITC

sc-393163 FITC
200 µg/ml
$336.00

Flk-1/KDR/VEGFR2 Antibody (D-8) PE

sc-393163 PE
200 µg/ml
$349.00

Flk-1/KDR/VEGFR2 Antibody (D-8) Alexa Fluor® 488

sc-393163 AF488
200 µg/ml
$364.00

Flk-1/KDR/VEGFR2 Antibody (D-8) Alexa Fluor® 546

sc-393163 AF546
200 µg/ml
$364.00

Flk-1/KDR/VEGFR2 Antibody (D-8) Alexa Fluor® 594

sc-393163 AF594
200 µg/ml
$364.00

Flk-1/KDR/VEGFR2 Antibody (D-8) Alexa Fluor® 647

sc-393163 AF647
200 µg/ml
$364.00

Flk-1/KDR/VEGFR2 Antibody (D-8) Alexa Fluor® 680

sc-393163 AF680
200 µg/ml
$364.00

Flk-1/KDR/VEGFR2 Antibody (D-8) Alexa Fluor® 790

sc-393163 AF790
200 µg/ml
$364.00

Flk-1/KDR/VEGFR2 (D-8) Neutralizing Peptide

sc-393163 P
100 µg/0.5 ml
$69.00

There is no signal using Flk-1(D8) on canine tissue - what kind of pretreatment would you suggest (Citrate? EDTA?); we already tried pH6, dil. 1:50-1:200

Asked by: Angelika2406
Thank you for your question. You can find pre-treatment options in our recommended protocol for IHC by following this link: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining We recommend heat treatment with 10 mM sodium citrate buffer, Pepsin, or Saponin. Please do not hesitate to get in touch with our technical service if these options do not improve your results.
Answered by: Tech Support Europe
Date published: 2019-07-09

We would like to get free sample of Flk-1 Antibody (D-8): sc-393163 antibody. Is it possible?

Asked by: martakiezun
Thank you for your question. Please get in touch with our technical service to arrange for a free sample of this antibody.
Answered by: Technical Support Europe
Date published: 2019-05-06

Is Flk-1 Antibody (D-8): sc-393163 applicable for flow cytometry analysis? 

Asked by: CLab
Thanks for your question. This antibody has not been tested for Flow cytometry and is therefore not recommended for that use. It may work fine, but has not been tested. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2018-11-30

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-02-09
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Rated 5 out of 5 by from Works well for WBWorks well on neonatal mouse lungs at concentration of 30ug. Antibody dilution will be 1:250 for overnight at 4oC
Date published: 2024-12-13
Rated 4 out of 5 by from Great for WBIt is a good antibody in WB. It works in dilution 1:1000.
Date published: 2024-04-01
Rated 3 out of 5 by from Not always convincingTested with different protocols on canine tissue (hemangiosarcoma), the results were not always convincing.
Date published: 2022-06-08
Rated 1 out of 5 by from Did not work for 1:500 dilutionTried 1:1000 and 1:500 dilution in human bladder cancer cell lines - no signal detected by WB analysis
Date published: 2018-04-03
Rated 4 out of 5 by from Good antibodyI detected great bands in mouse endothelial cells. Though, there was a bit of background, but nothing to affect my experiment.
Date published: 2017-10-24
Rated 5 out of 5 by from Effect is Quite GoodWhen we used Flk-1 Antibody in our aim cell, the results show is very good. The antibody has very good activity and improves the detecting sensitivity.
Date published: 2017-10-21
Rated 5 out of 5 by from Good for WB1:300 diluted for wb of rat protein ,the band is strong and special!
Date published: 2017-08-11
Rated 5 out of 5 by from Excellent antibody for Western blot.We obtained great results with this excellent antibody (working dilution 1:1000, blocking buffer: 5%no-fat dry milk) on rat brain and retina. It clearly detects three strong bands between 150 and 230 kDa, corresponding to immature, intermidiate glycosylated and mature glycosylated forms of Fkl1 (VEGFR2).
Date published: 2017-07-14
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Flk-1/KDR/VEGFR2 Antibody (D-8) is rated 4.1 out of 5 by 13.
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