Anti-Flk-1/KDR/VEGFR2 Antibody (A-3) recommended for detection of VEGFR2 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-Flk-1/KDR/VEGFR2 Antibody (A-3): sc-6251

Anti-Flk-1/KDR/VEGFR2 Antibody (A-3) is rated 4.2 out of 5 by 23.
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Datasheets

    • Flk-1/KDR/VEGFR2 Antibody (A-3) is a mouse monoclonal IgG1 κ, cited in 535 publications, provided at 200 µg/ml
    • raised against amino acids 1158-1345 mapping at the C-terminus of VEGFR2 of mouse origin
    • Anti-Flk-1/KDR/VEGFR2 Antibody (A-3) is recommended for detection of VEGFR2 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-Flk-1/KDR/VEGFR2 Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-6251 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Flk-1/KDR/VEGFR2 (A-3): sc-6251.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Flk-1/KDR/VEGFR2 Antibody (A-3). This work is in progress.
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Hi, is this antibody recommended for IF for detection on frozen human samples ? I am confused because it has been raised against mouse and I cannot find any citations on humans. thank you

Asked by: jackie2222
Thank you for your question. Yes, this antibody is reactive with mouse, rat and human Flk-1. There is data published on our website in formalin-fixed, paraffin-embedded human colon carcinoma tissue. This antibody has been cited in the literature 400 times, and many of those citations are also using this antibody for IHC or IF on human tissues, including frozen tissue sections.
Answered by: Technical Support
Date published: 2019-03-07

Is Flk-1 Antibody (A-3): sc-6251 usable for flow cytometry analysis? The datasheet has conflicting statements.

Asked by: CLab
Thank you for your question. This conjugated forms of this antibody, sc-6251, is warrantied for FCM. We do have publications for flow cytometry with this antibody, you can find them here: https://www.scbt.com/scbt/misc/citationResults.jsp?_requestid=4268173 If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2018-11-30

I need to use an antibody to detect the protein in samples of equine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 3
Date published: 2016-12-21

I need to use an antibody to detect the protein in avian samples. Do you know if this monoclonal antibody will work?

Asked by: DefinitelyNotMatt
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 9
Date published: 2016-12-15

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-15
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Rated 3 out of 5 by from Worked for WB analysis Tried 1:500 dilution. Signal detected for human bladder cancer cells but not canine.
Date published: 2018-06-19
Rated 5 out of 5 by from Gute Ergebnisse IHC-Paraffin Habe den AK 1:100 in Paraffinschnitten eingesetzt mit guten Ergebnissen.
Date published: 2018-02-14
Rated 5 out of 5 by from Working good for WB I got sample antibody to check expression of VEGFR2 after over-expression in HEK cells. I got good results with a sharp clear band of VEGFR2. I may purchase this antibody for my further experiments. The antibody dilution at 1:500 works better for WB
Date published: 2017-12-17
Rated 5 out of 5 by from Western blot This antibody had good expression in western blot. ( cell line: huvec)
Date published: 2017-11-22
Rated 3 out of 5 by from IF signal is not specific. IF (1:1000) on Cryosection from 4%PFA fixed E13.5 mouse cerebellum, signal is not specific.
Date published: 2017-11-08
Rated 3 out of 5 by from Protein is detected on different size. I tried several times, but protein was detected in 50kda size. The protein sample was extracted from endothelial cells of the human. The band appeared clearly but I am not sure whether it is correct.
Date published: 2017-10-10
Rated 5 out of 5 by from monoclonal I tried this antibody after trying dilution 1:1000, It's specific and reliable
Date published: 2017-05-19
Rated 5 out of 5 by from Excellent and well-cited antibody The Flk-1 Antibody (A-3) shows great staining pattern for IF and IHC applications with well over 100 publication citations for verification.
Date published: 2016-12-23
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